Gene: acriflavine resistant-2 III
acriflavine resistant-2
locus: acr-2
locus_name: acriflavine resistant-2
organism_type: B
chromosome_number: III
chromosome_side:
link_group: III
cultural_requirements: III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.
enzyme_id: 202
Enzyme: none
anid_name:
near_l: thi-4 (0/286)
near_r: sc (3 to 6%) and spg (1 to 11%)
neu_name:
supplement:
image_url:
Strains
References
