FGSC #8108

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Reporting Genes: fr ;acr-2

Species: crassa

Allele: P4445, KH5

Alternate Strain Number: 4433-2

Depositor: DDP

Linkage Group: IL, III

Mating Type: a

Species Number: 10

Opposite Mating Type: 8107

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
fr	IL. Between ro-10 (18%) and un-5 (6%) (798, PB). (789)Delicate branching on agar surface and delicate aerial growth with no conidia (789). Multiple hyphal branching (382). Deficient in glucose- 6-phosphate dehydrogenase (as are col-2 and balmutants) (949, 952). Partially deficient in linolenic acid (115); morphology partially corrected by exogenous linolenic acid (892, 943). Low adenylate cyclase activity and low adenosine 3',5'-phosphate (943, 950). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Unlike cr-1, fr is not corrected morphologically by exogenous cyclic nucleotides (892, 951). Scott (943) reported that morphology is corrected by theophylline; Rosenberg and Pall (892) reported no correction by phosphodiesterase inhibitors. Cell wall analysis; photograph (112, 278, 946). Reduced amount of cell wall peptides (1165). Recessive in duplications (808). Female sterile. Both known alleles (B110 and R2499) revert to fr+.	IL	B