FGSC #4196

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Reporting Genes: acr-2 uvs-4 leu-1

Species: crassa

Allele: KH5(r) ALS12 33757

Alternate Strain Number: M1390 A

Depositor: EK

Linkage Group: IIIR

Mating Type: A

Species Number: 10

ref1: Kafer 1982. Neurospora Newsl. 29:41-44, https://doi.org/10.4148/1941-4765.1645

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
uvs-4	IIIR. Left of ad-4 (4%) (932). Sensitive to UV (273, 932, 933) and histidine (932). Moderately sensitive to methyl methane sulfonate (537). Insensitive or only slightly sensitive to nitrosoguanidine (537, 933); no increased sensitivity to ionizing radiation (940) or mitomycin C (195, 537). Probably normal spontaneous mutation (275). Reduced UV-induced mutation (273). For mutation induction by other agents, see reference 940. Homozygous fertile; no effect on intragenic or intergenic recombination. Ascospore viability and early growth severely impaired in homozygous crosses (932). Recessive in heterokaryons (979).	IIIR	B