FGSC #7077

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Reporting Genes: Sk-3 acr-2

Species: crassa

Allele: P KH5(?)

Alternate Strain Number: 3278

Depositor: BCT

Linkage Group: III III

Mating Type: a

Species Number: 10

Genetic Background: OR

ref1: Turner & Perkins 1991. Am. Nat. 137:3, https://www.jstor.org/stable/2462576

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
Sk-3	Near the centromere. The killer allele Sk-3K suppresses crossing over in the interval r(Sk-2)-1-leu-1 (29% in controls). Recombines with his-7 (11%) but not with leu-1 (0/72) or acr-7 (0/60,000) and only 3/19,000 with acr-2. (1092; B.C. Turner, personal communication) Origin and characteristics resemble those of Sk-2, q.v. However, Sk-3K strains are sensitive to killing by Sk-2K and vice versa. The killer allele was found in N. intermedia, introgressed into N. crassa. Most wild-type strains of both species are sensitive, but resistant strains of N. intermedia have been found. Strains resistant to Sk-3K are not necessarily resistant to Sk-2K (857, 1092).		B