FGSC #877

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Reporting Genes: acr-2

Species: crassa

Allele: KH2(r)

Alternate Strain Number: 301

Mutagen: S

Depositor: KSH

Linkage Group: IIIL

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 878

ref1: Hsu K.S. Neurospora News Letter 1:5, 1962 H139; https://doi.org/10.4148/1941-4765.2082

ref2: Hsu 1963. J. Gen. Microbiol. 32:341-347, https://doi.org/10.1099/00221287-32-3-341; https://www.genetics.org/content/genetics/47/8/937.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B