FGSC #6832

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Reporting Genes: In(IL;IR)OY323;arg-5;acr-2;psi-1;at;ylo-1;wc-1

Species: crassa

Allele: OY323;27947;KH5;34C(t);M111;Y30539y;P829

Alternate Strain Number: 9368

Depositor: DDP

Linkage Group: all

Mating Type: A

Species Number: 10

Opposite Mating Type: 6833

ref1: Perkins Fungal Genet Newsl 37, https://doi.org/10.4148/1941-4765.1484

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
In(IL;IR)OY323			B
wc-1	VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).	VIIR	B
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
arg-5	Uses ornithine, citrulline or arginine.	IIR	B
at	Grows on minimal media. Conidia form in clumps, especially at the top of a slant.	V	B
psi-1	IVR. Right of the centromere (D.R. Stadler, A.M. Towe, and M. Loo, cited in reference 619). Left of T(ALS159) and pyr-1 (4%) (808, PB). Conidial germination and hyphal growth inhibited at 35°C but normal (or nearly so) at 20°C. Protein synthesis reduced after shift to restrictive temperature. Recessive in heterokaryons. (619) Scored as an irreparable heat- sensitive unmutant (see un).	IVR	B