FGSC #5194

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Reporting Genes: acr-2;cys-10 pdx-1 pan-1 uvs-2

Species: crassa

Allele: KH5(r) 39816 37803 5531 no#

Alternate Strain Number: M1990

Depositor: EK

Linkage Group: III;IVL IVR IVR IVR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B
uvs-2	IVR. Right of cys-4 (5%) (1023). Left of pmb (8%) (S. Ogilvie-Villa, cited in reference 248; R. Sadler and S. Ogilvie-Villa, personal communication) and the T(S4342) right breakpoint. Linked to T(AR209), T(T54M50), and T(ALS179) (2 to 6%), which mark the IVR tip (808). Sensitive to UV (273, 1023), ionizing radiation (537, 935, 940), methyl methane sulfonate (536, 537), nitrosoguanidine (509, 935), mitomycin C (537), 4-nitroquinoline 1-oxide (509), nitrous acid (D.R. Stadler and E. Crane, personal communication), and ICR-170 (509). Slight or no sensitivity to histidine (537, 759). No dimer excision (1164). Normal spontaneous mutation (275). High UV-induced mutation rate (273); for mutation induction by other agents, see references 509 and 940. Homozygous fertile; no effect on meiosis or crossing over. Recessive in heterokaryons (1023). uvs-2 is the most UV sensitive of Neurosporamutants (15 to 20 times wild type) (537, 938). Used to show that DNA repair is induced by a small dose of UV (1022). Used to demonstrate postreplication repair (130). Only known allele was discovered in several Seattle stocks of mixed ancestry, and thus may be present in lab stocks elsewhere (1023; D.R. Stadler, personal communication). Not to be confused with a cytoplasmically determined UV-sensitive mutation called uvs-2 in reference 187, but now called [uvs(cyt)].	IVR	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
cys-10	IVL. Left of acon-3 (1 to 6%), ace-4 (19 to 33%), and cut (28 to 37%) (578, PB). (721) Uses cysteine, cystathionine, homocysteine, or methionine, with a slight response to thiosulfate (469, 596, 721); however, E. Kafer (personal communication) found good growth on thiosulfate. Growth is better on casein hydrolysate than on methionine (D.D. Perkins, unpublished data). cys-10 chol-1double mutants grow better on methionine alone than on methionine plus choline (721). Lacks sulfite reductase, as do cys-2 and cys-4mutants (596). Formerly called met-4; see reference 721.	IVL	B
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B