FGSC #7159

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Reporting Genes: acr-2 spg

Species: crassa

Allele: KH5 no#

Alternate Strain Number: 3321

Depositor: DDP

Linkage Group: III III

Mating Type: a

Species Number: 10

Opposite Mating Type: 7158

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
spg	III. Between acr-2 (1 to 11%) and ser-1 (896). Linked to sc (<1%) and thi-4(0/103) (816, P13). Conidiating spreading colonies. Morphology distinct from that of scmutants. Good viability. Growth on minimal medium of Vogel (1103) is less spreading than on crossing medium of Westergaard and Mitchell (1134) (PB). Hyphae fuse to form bundles (382). Reduced amount of cell wall peptides (1165).	III	B