FGSC #6929

Reporting Genes: Sk-2K acr-2 (Sk from N. intermedia)

Species: crassa

Allele: Java KH5(r) Utilization may require PIC/MAT re Nagoya Protocol Check https://absch.cbd.int/

Alternate Strain Number: 3214

Depositor: BCT

Linkage Group: IIIL L

Mating Type: a

Species Number: 10

Opposite Mating Type: 6928

ref1: Campbell and Turner 1987. Genome 29:129, https://doi.org/10.1139/g87-022

ref2: https://doi.org/10.1128/mr.46.4.426-570.1982

Genes

Locus	Cultural Requirements	Link Group	Type
acr-2	III. Linked to thi-4 (0/286). Left of sc (3 to 6%) and spg (1 to 11%) (498, 816). acr-2 has been shown left of the centromere on published maps but without direct evidence. acr-2and trp-1 (on IIIR) cosegregated at the second division in 1 of 13 asci (H.B. Howe, Jr., personal communication), which would favor a right arm location. Resistant to acriflavine (494, 495); also resistant to 3-amino-1,2,4-triazole (seven alleles tested) (494). Resistance is probably dominant (heterokaryon tests) (498). Not resistant to malachite green. An excellent stable marker, fully fertile, with unambiguous scoring. Sizable inocula should be used to avoid false-negative tests. Use acriflavine at 50 µg /ml in minimal agar medium (816) (higher concentrations may be used) and aminotriazole at 0.5 mg/ml; both added before autoclaving.	III	B
Sk-2K			B

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