Gene: cycloheximide resistant-2 VR
cycloheximide resistant-2
locus: cyh-2
locus_name: cycloheximide resistant-2
organism_type: B
chromosome_number: V
chromosome_side: R
link_group: VR
cultural_requirements: VR. Right of lys-2 (<1%). Left of leu-5 (<1 to 2%) and sp(2 to 9%) (496, 818, PB). Resistant to cycloheximide (496, 748). Protein synthesis on mutant ribosomes proceeds in the presence of cycloheximide in a cell-free system (834). Excellent marker. Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium or with 1 µg added after autoclaving. Resistance in heterokaryons has been reported to be dominant (496, 626) or recessive (939); it may depend on nuclear ratios or media. Used in mutagenicity test systems (626). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Double mutant cyh-1;cyh-2grows slowly and is much more insensitive to cycloheximide than either single mutant (496).
enzyme_id: 202
Enzyme: none
anid_name:
near_l: lys-2 (<1%)
near_r: leu-5 (<1 to 2%) and sp(2 to 9%)
neu_name:
supplement:
image_url:
Strains
References
