FGSC #8406

Reporting Genes: cpc-3::hph; cyh-2

Species: crassa

Alternate Strain Number: SA1-2

Mutagen: KO

Depositor: ES

Linkage Group: V

Species Number: 10

Genetic Background: SL

ref1: Sattlegger et al 1998 J. Biol Chem. 273:20404-20416, https://doi.org/10.1074/jbc.273.32.20404

Genes

Locus	Cultural Requirements	Link Group	Type
cyh-2	VR. Right of lys-2 (<1%). Left of leu-5 (<1 to 2%) and sp(2 to 9%) (496, 818, PB). Resistant to cycloheximide (496, 748). Protein synthesis on mutant ribosomes proceeds in the presence of cycloheximide in a cell-free system (834). Excellent marker. Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium or with 1 µg added after autoclaving. Resistance in heterokaryons has been reported to be dominant (496, 626) or recessive (939); it may depend on nuclear ratios or media. Used in mutagenicity test systems (626). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Double mutant cyh-1;cyh-2grows slowly and is much more insensitive to cycloheximide than either single mutant (496).	VR	B
cpc-3	4.2% to cyh-2	V	B
cpc-3::hph			B

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