FGSC #9010

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Reporting Genes: cpc-1 cpc-3 cyh-2

Species: crassa

Alternate Strain Number: SA9-18

Depositor: MS

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cpc-3	4.2% to cyh-2	V	B
cyh-2	VR. Right of lys-2 (<1%). Left of leu-5 (<1 to 2%) and sp(2 to 9%) (496, 818, PB). Resistant to cycloheximide (496, 748). Protein synthesis on mutant ribosomes proceeds in the presence of cycloheximide in a cell-free system (834). Excellent marker. Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium or with 1 µg added after autoclaving. Resistance in heterokaryons has been reported to be dominant (496, 626) or recessive (939); it may depend on nuclear ratios or media. Used in mutagenicity test systems (626). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Double mutant cyh-1;cyh-2grows slowly and is much more insensitive to cycloheximide than either single mutant (496).	VR	B
cpc-1	VI. Right of ylo-1 (1 to 3%) (238). Affects simultaneously both ability to derepress and basal levels of enzymes in arginine and other amino acid biosynthetic pathways. cpc-1 mutations interfere with cross-pathway control of amino acid biosynthetic enzymes. Sensitive to 3-amino-1,2,4-triazole. Isolated as arginine auxotrophs by selection in the mutant arg-12s, q.v. The cpc-1;arg-12+ single mutant is prototrophic. Delayed growth after ascospore germination; the delay is not alleviated by arginine or precursors. Scorable by delayed growth. (61, 238) Exemplified by alleles CD-15 and CD-55 in reference 238 and j-2, j-5, and j-9 in reference 61.	VI	B