FGSC #7168

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Reporting Genes: cyh-2 leu-5

Species: crassa

Allele: KH53 45208

Alternate Strain Number: 5289

Depositor: DDP

Linkage Group: VR VR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cyh-2	VR. Right of lys-2 (<1%). Left of leu-5 (<1 to 2%) and sp(2 to 9%) (496, 818, PB). Resistant to cycloheximide (496, 748). Protein synthesis on mutant ribosomes proceeds in the presence of cycloheximide in a cell-free system (834). Excellent marker. Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium or with 1 µg added after autoclaving. Resistance in heterokaryons has been reported to be dominant (496, 626) or recessive (939); it may depend on nuclear ratios or media. Used in mutagenicity test systems (626). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Double mutant cyh-1;cyh-2grows slowly and is much more insensitive to cycloheximide than either single mutant (496).	VR	B
leu-5	VR, Between cyh-2 (1%) and sp (3 to 9%) (818, 839, PB). (812)Strains carrying the only auxotrophic allele, 45208t. have a partial leucine requirement at low temperatures and a tighter leucine requirement at 34°C and stop growth at 37 to 39 C, regardless of leucine supplementation (290, 839). Altered leucyl-tRNA synthetase (839). Apparently a gene complex consisting of structural genes for cytoplasmic leucyl-tRNA synthetase and a separate mitochondrial leucyl-tRNA synthetase. Mutations mapping in the leu-5 region can affect either enzyme separately or both simultaneously (69, 431). In the [cni-3] mitochondrial mutant, mitochondrial tRNA synthetase is greatly increased, whereas the cytoplasmic enzyme is unchanged (430). Allele 45208t causes alterations in unrelated enzymes, apparently via mistranslation (607, 839). Used to study the hypothesis that senescence is due to faulty protein synthesis (607). Assembly of glycerolkinase and glycerol-3-phosphate dehydrogenase into inner mitochondrial membrane is not impaired in leu-5 strains (213). Poor recovery from ascospores at 34°C (complex complete medium); best germinated at 25°C. Allele 45208t is somewhat unstable (431).	VR	B