FGSC #2143

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Reporting Genes: pdx-1;scon cyh-2

Species: crassa

Allele: 37803;sconc;KH53(r)

Alternate Strain Number: sconc,act-2

Depositor: RLM

Linkage Group: IVR;VR VR

Mating Type: a

Species Number: 10

ref1: Burton & Metzenberg J. Bact 109:140-151 1972, https://doi.org/10.1128/jb.109.1.140-151.1972

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cyh-2	VR. Right of lys-2 (<1%). Left of leu-5 (<1 to 2%) and sp(2 to 9%) (496, 818, PB). Resistant to cycloheximide (496, 748). Protein synthesis on mutant ribosomes proceeds in the presence of cycloheximide in a cell-free system (834). Excellent marker. Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium or with 1 µg added after autoclaving. Resistance in heterokaryons has been reported to be dominant (496, 626) or recessive (939); it may depend on nuclear ratios or media. Used in mutagenicity test systems (626). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Double mutant cyh-1;cyh-2grows slowly and is much more insensitive to cycloheximide than either single mutant (496).	VR	B
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B
scon	Probably VR, right of his-6 (R.L. Metzenberg, personal communication). But presence of a translocation (and, therefore, pseudolinkage) cannot be ruled out because white spores are prevalent in sconc crosses. Difficult to map because of infertility (R. L. Metzenberg, personal communication). Regulates a group of enzymes of sulfur metabolism, including arylsulfatase and choline sulfatase. Allele sconc is constitutive for these enzymes, but its effect in a heterokaryon is restricted to its own nucleus (123). Hypostatic to regulatory gene cys-3 (284). Review of regulation (642). Ascospores and conidia germinate poorly (123, 930).	VR?	B