THE FOUNDATION OF NEUROSPORA BIOLOGY
    What is Neurospora?
    Why choose Neurospora?
    What is a model organism?
    The NIH Neurospora/model organism page is no longer available

AN INTRODUCTION TO NEUROSPORA RESEARCH
    Taxonomic classification
    Life cycles
    Basic cell structure
    Neurospora crassa dimensions at 25 C
    Ecology / Biogeography
    Phylogeny
    Genome basics
    Gene nomenclature
    Some current areas of research
    Useful reviews, reference works, and compilations
    Neurospora Awards
    E-news Archive

NEUROSPORA GENETIC DATABASES
    The Neurospora genome
    SNP map for Mauriceville vs OakRidge

    The Neurospora E- compendium
          The 2001 Neurospora compendium
          The 1982 Neurospora compendium
    Neurospora genetic maps
    The Neurospora Bibliography
    Textpresso Fungal Literature Databases

STRAINS AND OTHER BIOLOGICAL MATERIALS
    The FGSC Database Search page
    Neurospora strains
    Molecular materials
 

EXPERIMENTAL METHODS
    Neurospora online protocol guide
    Overview of techniques
    Details of basic protocols.
    

IDEAS FOR STUDENT LABS
    FGSC website on student labs
    Single gene inheritance/life cycle
    The use of the spot test
    Segregation analysis and epistasis
    Miscellaneous ideas for labs
    Neurospora on a budget

PHOTO GALLERIES
    Morphological mutants and wild-types
    Neurospora Sexual Biology

Information about Neurospora

 

 

 

 

 

 


False color rendition by Tony Griffiths of a photograph of Neurospora ascospores taken by Namboori Raju

Knockout mutants

     Individual knockout mutant strains individually or in sets.

     Arrayed mutants  

     Completed knockouts are described at the Dartmouth web-site with a complete cross referencing list. 

     We have over 130 plates of mutants available as frozen conidia in glycerol in 96-well plates.
     We are asking $100 per plate (plus shipping) with a cap of $3000 for people who want the entire set.

The Knockouts are described here:
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.
Colot HV, Park G, Turner GE, Ringelberg C, Crew CM, Litvinkova L, Weiss RL, Borkovich KA, Dunlap JC.
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10352-7

          All strains are in the FGSC 4200/ FGSC 2489 (St. Lawrence) genetic background.
         
They are listed together here
 

cDNA libraries of Mauriceville (FGSC 2225) from the Functional Genomics Program

SNP primer sets are available and are described in Genetics 181:767-781
We have frozen primers in 96 well format as sent from IDT and can send them frozen on dry ice.
Primer Identity Spreadsheet (xls)


 


N. crassa mutant strains being characterized by whole genome sequence analysis

 

Bob Metzenberg memorial       
David D. Perkins memorial

Perkins Obituary

 

 


The Neurospora home page is a collaborative effort of Drs. A.J.F. Griffiths of the University of British Columbia and Kevin McCluskey at the Fungal Genetics Stock Center


Return to the FGSC main page

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