(Note: all the mutant alleles and strains referred to can be found in the FGSC stock list.)
1. Screening for morphological mutants (after UV or nitrous acid mutagenesis).
2. A simple complementation test (al-1 aux-1 + al-2 aux-2). Aux represents any auxotrophic allele.
3. Using the per mutation to deduce the origin of the perithecial wall. (barrage in cross fl x fl per)
4. Reversion of ad-3 base pair transition mutations
5. Ascus patterns in crosses of normal x rearrangements unknown to the student.
6. Senescence “lifespans” in kalilo plasmid-containing strains, measured by serial subculturing.
7. Reconstructing the arginine pathway. (See G J Stein 1973 Laboratory Exercises in Genetics, Collier-Macmillan, New York, for FGSC strains and methods.)
8. Effect of temperature on growth of cot-1.
9. Selection for methyl tryptophan resistance. (This is an easy selection for forward mutations at one gene locus mtr.)
10.Suppressors of morphological mutations.
11. The mitochondrial mutant poky: crosses and cytochrome spectra. (See The Experimental Geneticist by P Saint Lawrence et al., 1974, W H Freeman & Co new York.)