Neurospora crassa arrayed knockout mutant plates

As of 2015, sets of arrayed mutants are not available. The FGSC does not have grant support, nor is there revenue potential to re-generate these sets. Replica plating with available resources has not proven to be reliable with cross-contamination very common. If you already paid for a full set and obtained plates before the whole set was arrayed, you may obtain the plates remaining by paying for shipping

Master Plate data sheet - Excel - plates 1 -122,  10/2/2012. Full strain lists are available below

Important notes about the arrayed strains:

Some strains are in the plates multiple times. Please see this list

Please validate strains by PCR prior to publication. If you have any concern about strains from the arrayed set, please ask for the original mutant.

If you use a Neurospora knockout strain from the functional genomics program, you should cite :

Colot HV, Park G, Turner GE, Ringelberg C, Crew CM, Litvinkova L, Weiss RL, Borkovich KA, Dunlap JC.
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.
Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10352-7.  Also, you may acknowledge  use of materials from the
Neurospora Functional Genomics grant P01GM68087

There is a known issue where soft phenotypes are present in some mutants and these soft phenotypes segregate separately from the deletion cassette. Available data listing these strains is online here

The following strains were recalled by the Riverside lab as being incorrect according to Southern Hybridization:

FGSC# NCU Plate Well
11459 ncu00340 4 1A
11460 ncu01345 4 2A
11461 ncu01459 4 3A
11462 ncu04050 4 4A
11466 ncu06182 4 8A
12469 ncu03833 15 12C
15793 ncu03833 21 1B
11290 ncu01613 1 6H
11291 ncu01613 1 7H

Additional strains that are of questionable authenticity.

FGSC # Notes
13805, 13806 Includes NCU05600 and NCU12096

10172

NOT AVAILABLE

11290

NCU01613.2 awaiting replacement, NOT AVAILABLE

11291

NCU01613.2 awaiting replacement, NOT AVAILABLE

11397

NCU00090.2 NOT AVAILABLE

11436

NOT AVAILABLE replaced by strain 17933

11459

NCU00340.2 Het-NOT AVAILABLE, Southern wrong replaced with 21335

11460

NCU01345.2 Het-NOT AVAILABLE- Southern wrong

11461

NCU01459.2 Het-NOT AVAILABLE- Southern wrong

11462

NCU04050.2 Het-NOT AVAILABLE- Southern wrong

11466

NCU06182.2 het-NOT AVAILABLE- Southern wrong replaced by 18201

11549

NOT AVAILABLE

11550

NOT AVAILABLE

11656

NCU00685.2 Heterokaryon (hrr25) (NOT AVAILABLE)

12469

NOT AVAILABLE

12512

NOT AVAILABLE (cnb-1)

15742

NCU00434.2 This strain is incorrect. NOT AVAILABLE.

15793

NOT AVAILABLE

16007

NCU09298.2 heterokaryon NOT AVAILABLE (new annotation info)

16571

NCU02537.2 NOT AVAILABLE(new annotation info)

17888

NCU07008.2 (cao-1) this strain is incorrect, NOT AVAILABLE

17935

NCU01187.2 NOT AVAILABLE (so mutation) (cpc-3)

17962

NCU07172.2 (stk-8) so mutation NOT AVAILABLE

17966

NCU07880.2 NOT AVAILABLE (so mutation) (prk-6)

18951

NCU08485.2 NOT AVAILABLE, unable to revive

20904

NCU02208.2 This strain is incorrect, NOT AVAILABLE

20905

NCU02208.2 This strain is incorrect, NOT AVAILABLE

12752 NCU03006.2 (erg-4) (a client reports the ability to amplify the erg-4 gene in this strain)
12753 NCU03006.2 (erg-4) (a client reports the ability to amplify the erg-4 gene in this strain)

 


 

Special function sets
Hyphal Growth Set Excel
Transcription Factor Set
Plates 1 and 2
Plate 3 (December 2006)

Html   Excel
Excel
Kinase (the first plate repeats strains elsewhere in the genome set, the second kinase plate is plate 113) Excel, Plate 113

 

Individual plate listings
Plate Number FGSC #'s Strain locations
Plate 1 11201-11296 Excel
Plate 2 11297-11392 Excel
Plate 3 11393-11458 Excel
Plate 4 11459-11526 11668-11689 Excel
Plate 5 11097-11147 Excel
Plate 6 11001-11096 Excel
Plate 7 11690-11788 Excel
Plate 8 11789-11890 Excel
Plate 9 11891-11988 Excel
Plate 10 11528-11637 Excel
Plate 11 11638-11667, 11148-11200 Excel
Plate 12 11989-12086 Excel
Plate 13 12335-12433 Excel
Plate 14 12087-12184 Excel
Plate 15 12434-12530 Excel
Plate 16 12185-12284 Excel
Plate 17 12285-12334, 12531-12576 Excel
Plate 18 12581- 12680 Excel
Plate 19 15670- 15770 Excel
Plate 20 12681- 12776 Excel
Plate 21 15771- 15869 Excel
Plate 22 12777- 12874 Excel
Plate 23 15726 - 15969 Excel
Plate 24 12799 - 12968 Excel
Plate 25 15970 - 16068 Excel
Plate 26 12969 - 13070 Excel
Plate 27 13071 - 13166 Excel
Plate 28 15996, 16070 - 16170 Excel
Plate 29 Morphological Mutants Excel
Plate 30 13167-13268 Excel
Plate 31 13269-13365 Excel
Plate 32 13366-13461 Excel
Plate 33 13463-13559 Excel
Plate 34 16171-16267 Excel
Plate 35 13393-13653 Excel
Plate 36 16268-16363 Excel
Plate 37 13654 - 13751 Excel
Plate 38 16364 - 16459 Excel
Plate 39 16460 - 16555 Excel
Plate 40 13752-13847 Excel
Plate 41 13846-13941 Excel
Plate 42 16556-16652 Excel
Plate 43 16653-16752 Excel
Plate 44 16749-16844 Excel
Plate 45 13942 -14037 Excel
Plate 46 14038-14133 Excel
Plate 47 16845-16940 Excel
Plate 48 16941-17036 Excel
Plate 49 14138-14233 Excel
Plate 50 14234-14329 Excel
Plate 51 14330-14425 Excel
Plate 52 14426-14521 Excel
Plate 53 14522-14617 Excel
Plate 54 17037-17132 Excel
Plate 55 17133-17228 Excel
Plate 56 17229- 17324 Excel
Plate 57 14618-14713 Excel
Plate 58 14714- 14809 Excel
Plate 59 14810-14905 Excel
Plate 60 17325-17420 Excel
Plate 61 14906-15001 Excel
Plate 62 15002-15097 Excel
Plate 63 17421-17516 Excel
Plate 64 15098-15195 Excel
Plate 65 17517-17612 Excel
Plate 66 15196-15291 Excel
Plate 67 15292-15387 Excel
Plate 68 17613-17708 Excel
Plate 69 14134-15479 Excel
Plate 70 17709-17804 Excel
Plate 71 15480-15575 Excel
Plate 72 17805-17900 Excel
Plate 73 15576-15669, 19672-19673 Excel
Plate 74 19674-19769 Excel
Plate 75 19770-19865 Excel
Plate 76 17902-17999 Excel
Plate 77 19866-19961 Excel
Plate 78 19962-20057 Excel
Plate 79 17999-18094 Excel
Plate 80 18095-18190 Excel
Plate 81 20058-20153 Excel
Plate 82 17901-20247 Excel
Plate 83 18181-18286 Excel
Plate 84 20248-20343 Excel
Plate 85 18287-18382 Excel
Plate 86 19034-19129 Excel
Plate 87 18383-20346 Excel
Plate 88 19130-19225 Excel
Plate 89 19005-19292 Excel
Plate 90 18476-20351 Excel
Plate 91 19293-19388 Excel
Plate 92 19389-20374 Excel
Plate 93 18567-18662 Excel
Plate 94 18663-18758 Excel
Plate 95 18759-18854 Excel
Plate 96 18855-18949, 18952 Excel
Plate 97 18952-19548 Excel
Plate 98 19549-19627, 18961-18977 Excel
Plate 99 18976-19004, 20375-20441 Excel
Plate 100 19628-19671, 20442-21029 Excel
Plate 101   Excel
Plate 102   Excel
Plate 103   Excel
Plate 104   Excel
Plate 105   Excel
Plate 106   Excel
Plate 107   Excel
Plate 108   Excel
Plate 109   Excel
Plate 110   Excel
Plate 110   Excel
Plate 111   Excel
Plate 112   Excel
Plate 113   Excel
Plate 114 21683 - 21794 Excel
Plate 115 21740, 21795 - 21889 Excel
Plate 116 21742, 21802, 21890 - 21983 Excel
Plate 117 21984 - 22081 Excel
Plate 118 22082 - 22185 Excel
Plate 119 22186 - 22286 Excel
Plate 120 22287 - 22388 Excel
Plate 121 22389 - 22506 Excel
Plate 122 22507 - 22626 Excel
Plate 123   Excel
Plate 124   Excel
Plate 125   Excel
Plate 126   Excel
Plate 127   Excel
Plate 128   Excel
Plate 129   Excel
Plate 130   Excel
Plate 131   Excel
Plate 132   Excel
Plate 133   Excel
Plate 134   Excel
Plate 135   Excel
Plate 136   Excel

Some plates span more than 96 strains either because there are missing strains, or some
strains have been segregated because they do not grow without special attention.

Strains that have not been placed in the main array series because they have morphological
defects will be segregated onto a Morphological mutant plate (eg, plate 29)

We prefer not to break up the arrayed sets and have made sets of strains that represent groups of
mutants.  Please do not ask for individual plates from the main series.
 

Special function plates (All of these strains are also in the main array series. )

Hyphal Growth Set 11202-11325  Excel
Transcription Factor Set,
plates 1 and 2
Plate 3 (December 2006)
11001- 11139

 

Html   Excel

Excel

Kinase (the first plate repeats strains elsewhere in the genome set, the second kinase plate is plate 113)   Excel, Plate 113

 


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7/23/13