FGSC #7394

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Reporting Genes: cum r(Sk-3) leu-1

Species: crassa

Allele: P5241 P 33757

Alternate Strain Number: 3331

Depositor: BCT

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
r(Sk-3)	IIIL. Between cum (4%) and acr-7 (17%). Prevents killing by Sk-3 of ascospores that are not themselves Sk-3. Does not confer resistance to killing by Sk-2. Allelism of r(Sk-3) with Sk-3 is not excluded because recombination in this region is blocked when Sk-3 is heterozygous. Recombination is not blocked by r(Sk-3). Found in Neurospora intermedia and introgressed into Neurospora crassa. For reference strains and tester strains for identifying the various killers and genes conferring resistance to killing, see refs. (2127) and (2128) in 2001 Neurospora compendium.	IIIL	B
cum	IIIL. Left of r(Sk-2) (4%), acr-7 (5 to 18%), and acr-2(18%). No recombination with Sk-2K. (PB; B.C. Turner, personal communication) Initially colonial; then spreads and sends up blooms of aerial hyphae which conidiate profusely at the shallow ends of agar slants. Good female fertility. Similar in morphology to the mutants sn and sp and to the mutant cot-4 at 25 C (PB).	IIIL	B