FGSC #7390

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Reporting Genes: cum Sk-3 his-7

Species: crassa

Allele: P5241 P Y152M31

Alternate Strain Number: 3288

Depositor: BCT

Linkage Group: III III III

Mating Type: A

Opposite Mating Type: 7391

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group
cum	IIIL. Left of r(Sk-2) (4%), acr-7 (5 to 18%), and acr-2(18%). No recombination with Sk-2K. (PB; B.C. Turner, personal communication) Initially colonial; then spreads and sends up blooms of aerial hyphae which conidiate profusely at the shallow ends of agar slants. Good female fertility. Similar in morphology to the mutants sn and sp and to the mutant cot-4 at 25 C (PB).	IIIL
his-7	IIIR. Between leu-1 (8 to 20%) and thi-2 (1 to 2%) (2199 1052, PB). (162, 1123)Requires histidine. Blocked before imidazole glycerol phosphate (162, 1123) (Fig. 14). Intralocus recombination (172).	IIIR
Sk-3	Near the centromere. The killer allele Sk-3K suppresses crossing over in the interval r(Sk-2)-1-leu-1 (29% in controls). Recombines with his-7 (11%) but not with leu-1 (0/72) or acr-7 (0/60,000) and only 3/19,000 with acr-2. (1092; B.C. Turner, personal communication) Origin and characteristics resemble those of Sk-2, q.v. However, Sk-3K strains are sensitive to killing by Sk-2K and vice versa. The killer allele was found in N. intermedia, introgressed into N. crassa. Most wild-type strains of both species are sensitive, but resistant strains of N. intermedia have been found. Strains resistant to Sk-3K are not necessarily resistant to Sk-2K (857, 1092).