FGSC #7198

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Reporting Genes: arg-12 fl rip-1

Species: crassa

Allele: UM107 P 4M

Alternate Strain Number: 2203

Depositor: DDP

Linkage Group: IIR IIR IIR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
rip-1	IIR. Linked to un-15 (1%); right of fl and trp-3 (6 to 9%). (PB). Conditional defect in production of 60S ribosomal subunits (622). At the restrictive temperature (37 C), RNA synthesis is affected first and then protein synthesis; 60S cytosolic ribosomal subunits are underaccumulated, and relatively little 25S rRNA is produced (618, 620, 622; P.J. Russell, personal communication). Defective ribosome biosynthesis at high temperatures is attributed to a defect in rRNA processing (622). Attains 2.4% of the wild-type growth rate at 35°C and 80% at 25°C (P.J. Russell, personal communication). Scored as an irreparable heat-sensitive un mutant (see un). Good fertility, growth, and viability make rip-1preferable to un-15 as a marker for the right end of II. The onginal strain carrying both rip-1 and inl (89601) (382, PB). was called 4M(t). The rip-1 allele in this strain was originally called 1(t).	IIR	B
arg-12	Uses arginine or citrulline.	IIR	B