FGSC #6885

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Reporting Genes: cys-10 pmb

Species: crassa

Allele: 39816 cnr-37(r)

Alternate Strain Number: 4272

Depositor: DDP

Linkage Group: IVL IVR

Mating Type: a

Species Number: 10

Genetic Background: sl

Opposite Mating Type: 6884

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pmb	IVR. Right of uvs-2 (8%) (S. Ogilvie-Villa, cited in reference 248; R. Sadler and S. Ogilvie-Villa, personal communication). Defective in basic L-amino acid transport (system III as defined in reference 778); reduced uptake of L-arginine, L-lysine, and L-histidine (778, 1152, 1076). Used extensively for transport studies; see reference 1150. Altered surface glycoprotein (1038). pmb mutants selected as resistant to L-canavanine (889, 1152). Allelic with bat (R. Sadler and S. Ogilvie-Villa, personal communication), which was selected in arg-12s;pyr-3 (CPS- ACT+) by ability to grow on minimal medium plus arginine, when the parental double mutant was not able to grow because of arginine uptake and feedback onto the arginine-specific carbamyl phosphate synthase (1074). Possibly allelic with basa, which was selected by the inability of the mutant his-3 to grow on histidine plus methionine (628). Probably allelic with bm-1 (linked to pyr-2, 24%), which was selected by canavanine resistance (913). Probably allelic with argR (565, 566), q.v. Called Cr-10, Pm-B, pm b, UM-535, and can-37. See Transport.	IVR	B
cys-10	IVL. Left of acon-3 (1 to 6%), ace-4 (19 to 33%), and cut (28 to 37%) (578, PB). (721) Uses cysteine, cystathionine, homocysteine, or methionine, with a slight response to thiosulfate (469, 596, 721); however, E. Kafer (personal communication) found good growth on thiosulfate. Growth is better on casein hydrolysate than on methionine (D.D. Perkins, unpublished data). cys-10 chol-1double mutants grow better on methionine alone than on methionine plus choline (721). Lacks sulfite reductase, as do cys-2 and cys-4mutants (596). Formerly called met-4; see reference 721.	IVL	B