permease basic amino acid

locus: pmb

locus_name: permease basic amino acid

organism_type: B

chromosome_number: IV

chromosome_side: R

link_group: IVR

cultural_requirements: IVR. Right of uvs-2 (8%) (S. Ogilvie-Villa, cited in reference 248; R. Sadler and S. Ogilvie-Villa, personal communication). Defective in basic L-amino acid transport (system III as defined in reference 778); reduced uptake of L-arginine, L-lysine, and L-histidine (778, 1152, 1076). Used extensively for transport studies; see reference 1150. Altered surface glycoprotein (1038). pmb mutants selected as resistant to L-canavanine (889, 1152). Allelic with bat (R. Sadler and S. Ogilvie-Villa, personal communication), which was selected in arg-12s;pyr-3 (CPS- ACT+) by ability to grow on minimal medium plus arginine, when the parental double mutant was not able to grow because of arginine uptake and feedback onto the arginine-specific carbamyl phosphate synthase (1074). Possibly allelic with basa, which was selected by the inability of the mutant his-3 to grow on histidine plus methionine (628). Probably allelic with bm-1 (linked to pyr-2, 24%), which was selected by canavanine resistance (913). Probably allelic with argR (565, 566), q.v. Called Cr-10, Pm-B, pm b, UM-535, and can-37. See Transport.

enzyme_id:

anid_name:

near_l: uvs-2 (8%)

near_r:

neu_name:

supplement:

image_url:

Strains

FGSC #	Reporting Genes	Species
FGSC #1683	pmb	crassa
FGSC #2275	pmb	crassa
FGSC #2276	mtr pmb	crassa
FGSC #2605	Has been replaced by strain number: 4607	crassa
FGSC #2606	Has been replaced by strain number: 4608	crassa
FGSC #2608	Has been replaced by strain number: 4608	crassa
FGSC #4246	pmn;pmb;pmg;lox	crassa
FGSC #4247	lox;pmn;pmb;pmg	crassa
FGSC #4607	pmn pmb;pmg	crassa
FGSC #4608	pmn pmb;pmg	crassa
FGSC #4810	pmb;pmg	crassa
FGSC #4811	pmb;pmg	crassa
FGSC #4814	pmb	crassa
FGSC #4815	pmb	crassa
FGSC #6884	cys-10 pmb	crassa
FGSC #6885	cys-10 pmb	crassa
FGSC #7924	pmb;aga	crassa
FGSC #7925	pmb;aga	crassa

References

Authors	Title	Year
De Busk, R. M. , and A. G. De Busk.	Physiological and regulatory properties of the general amino acid transport system of N. crassa.	1980
Koh, C. L.	Genetic control of the utilization of basic amino acids by their auxotrophs in Neurospora crassa.	1973
Koh, C. L. , and C. C. Ho.	Mutations affecting the utilization of basic amino acids in Neurospora.	1972
Magill, C. W. , H. Sweeney, and V. W. Woodward.	Histidine uptake in strains of Neurospora crassa with normal and mutant transportsystems.	1972
Pall, M. L.	Amino acid transport in Neurospora crassa. II. Properties of a basic amino acid transport system.	1970
Roess, W. B. , and A. G. DeBusk.	Properties of a basic amino acid permease in Neurospora crassa.	1968
Sanchez, S. , L. Martinez, and J. Mora.	Interactions between amino acid transport systems in Neurospora crassa.	1972
Stuart, W. D. and A. G. DeBusk.	Genetic alterations of ribonuclease-sensitive glycoprotein subunits of amino acid transportsystems in Neurospora crassa conidia.	1975
Thwaites, W. M.	A mutation reducing feedback regulation by arginine in suppressed pyr-3 mutants in Neurospora.	1967
Thwaites, W. M. , and L. Pendyala.	Regulation of amino acid assimilation in a strain of Neurospora crassa lacking basic amino acidtransport activity.	1969
Wolfinbarger, L. , Jr. , and A. G. DeBusk.	Molecular transport. I. In vivo studies of transport mutants of Neurospora crassa withaltered amino acid competition patterns.	1971