FGSC #5381

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Reporting Genes: T(VL->IL)QNS-6(OY321),nit-2 leu-3;caf-1 at

Species: crassa

Allele: OY321 nr37 R156;KH101 M111

Alternate Strain Number: 15-1535

Depositor: DDP

Linkage Group: IL L L;VL L

Mating Type: a

Species Number: 10

ref1: Perkins, et al 1986 Genetics 114:791-817, https://doi.org/10.1093/genetics/114.3.791

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nit-2	IL. Right of the T(39311) left breakpoint and of un-5(2%). Left of In(OY323) and leu-3 (12 to 18%) (57, 808, 816, PB). (335, 1135) Cannot use nitrate, nitrite, purines, or most amino acids as a nitrogen source but will grow on ammonia, glutamine, or glutamate. nit-2+ is a major nitrogen control gene and mediates nitrogen catabolite repression. The nit-2 mutant is missing (or has severely reduced levels of) nitrate reductase, nitrite reductase, uricase, xanthine dehydrogenase, allantoinase, allantoicase, L-amino acid oxidase, general amino acid permease, extracellular protease, and an intracellular neutral phenylmethylsulfonyl fluoride-sensitive protease (227, 324, 441, 872, 1001, and references therein). Also affects levels of glutamate dehydrogenases (226) and uptake of uracil and uridine (128). Prevents leaky growth of the mutant am on minimal medium (155). The product of the nit-2gene has been tentatively identified as a nuclear DNA-binding protein, whose affinity for DNA is reduced in the presence of glutamine (433). Allele K31 (called pink) originated in N. sitophila and was introgressed into N. crassa (335); protein product of K31 may show altered mobility (433). Recombination within the nit-2 locus is subject to regulation by rec-1 (157). Heterozygosity for closely linked ss reduces recombination within nit-2 (161). Called amr: ammonium regulation in reference 872.	IL	B
leu-3	IL. Right of the In(OY323) left breakpoint and nit-2 (12 to 18%). Left of cyt-1 (5 to 8%) and T(OY321) (57, 816, PB; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (868)Requires leucine (867, 868). Regulatory mutation; prevents synthesis of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase and prevents full derepression of alpha-isopropylmalate synthetase; also involved in regulation of isoleucine and valine synthesis, q.v. (427, 771, 833) (Fig. 15). The original allele, 47313, is leaky, but some other alleles, e.g., R156, are not.	IL	B
caf-1	VL. Right of T(OY321) (11%), T(AR30) (19%), and. hence, of NO. Left of T(AR33)(5 to 12%) and lys-1 (4 to 14%). (817, PB; K.S. Hsu, unpublished data) Resistant to caffeine (494). Resistance is dominant in duplications from T(AR33) (817). Scoring is clear at 25°C and poor at 34°C on slants with 2 or 2.5 mg of caffeine per ml of minimal medium without sorbose (R.L. Metzenberg, personal communication). Also readily scorable by using conidial suspensions spotted on plates containing 2 mg of caffeine per ml of minimal sorbose medium (PB).	VL	B
at	Grows on minimal media. Conidia form in clumps, especially at the top of a slant.	V	B
T(VL->IL)QNS-6(OY321)	Translocation		B