FGSC #2718

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Reporting Genes: qa-2;aro-9

Species: crassa

Allele: 326M237;Y325M6

Alternate Strain Number: 326M237.Y325

Depositor: MEC

Linkage Group: VIIR;IIR

Mating Type: A

Species Number: 10

Genetic Background: SL

ref1: Jacobsen et al 1975 J Bacteriol 124:491-496, https://doi.org/10.1128/jb.124.1.491-496.1975

ref2: Jacobsen et al 1975 J Biol Chem 250:6008-6014, https://doi.org/10.1016/S0021-9258(19)41150-2

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
qa-2	VII. In the qa cluster between qa-4 and met-7 (0.02%) (146). (884) Lacks catabolic dehydroquinase (148, 517, 884). Unable to use quinate or shikimate as the sole carbon source in the presence of aro-9, which results in the absence of biosynthetic dehydroquinase (885) (Fig. 21). aro-9;qa-2+ strains grow on minimal medium without supplement. qa-2 is conveniently scored as an aro auxotroph when aro-9 is present. qa-2+ cloned in pBR322 (pVK57) is expressed constitutively from its own promoter in E. coli(23, 388, 451); has been returned to Neurospora chromosomal sites by transformation (150). Allele M246 is stable (150).	VII	B
aro-9	Requires shikimic acid or a mixture of the four aromatic amino acids when combined with qa-1. Single mutant grows on minimal media.	IIR	B