Co-segregation data from Dr. S. Free

Below is a list of those wells in the KO library which contain protoperithecia-defective isolates in which the protoperithecia-defective phenotype does not cosegregate with the hygromycin insertion/KO mutation. An * in the well# column means that a second isolate of the other mating type exists in the library and the second isolate does not have the protoperithecia-defective phenotype. A # in the NCU# column means that our PCR assay suggests that the hygromycin mutation does not disrupt the indicated gene. The information in the cosegregation data column is given as the number of parental type progeny/total progeny. An XXX in the coseg. data column indicates that because the second isolate for the deletion strain was not affected in properithcia development, it was clear that the mutant phenotype was not due to the deletion and the strain was not tested. An ---- in the coseg data column indicates that the ratio of parental type progeny/total progeny was not recorded but that the strain was recorded as not showing co-segregation between the protoperithecia defective phenotype and hygromycin resistance.
 
Plate # well# NCU# coseg. data
1 A8* 01312 XXX
1 D11* 06650 -----
1 F4 09866 not recovered for second screen
1 G10* 00157 11/21 & 14/24
1 H1 00810 10/18
2 A5 07621 8/24
2 B9* 4096 -----
2 D9 07622 14/24
2 E11 3043 not recovered for second screen
3 A5 00090 13/24 but coseg morph phenotype
3 B3* 06366 11/23
3 D8 07024 contaminated well
3 D9 00019 16/24
4 E3 00117 17/23
4 H3/H4 07817 18/24
5 D4 04561 8/24
5 A12(ada-2) 02017 contaminated well (proto+)
6 E5(ada-5) 03931 14/23
6 E10(ada-4) 03320 12/24
6 F2(ada-7) 06068 12/24
6 G5* 03686 12/24
7 A1/A2 03800 14/24
7 A3/A4 02692 11/23
7 H4 03875 14/22 but coseg growth phenotype
8 D3* 02211 15/24
8 G7 02734 9/24
9 C10 07361 16/23
9 E10* 08739 14/24
10 C11 04145 15/24
10 H6* 05196 10/24
11 D3* 02795 16/24
11 G4* 06605 11/24
12 A8/A9 04350 10/24
12 A11* 00481 15/23
12 G1* 07381 10/24
13 B4* 09611 11/24
13 B12* 01143 11/24
13 E8* 00284 18/24
13 E10* 03850 12/24
13 E11 03854 11/22
13 F3* 03862 8/24
13 F12/G1 04583 15/24 & 16/24
13 H11 02534 9/24
15 A4 04078 10/23
15 B2 09432 12/22
15 B8/B9 08301 ------
16 B5 00159 not recovered for second screen
16 E6* 01062 13/24
17 C4* 06710 16/24
17 F2 05157 12/24
17 F9/F10 02354 13/24 but coseg growth phenotype 24/24
17 G1* 06112 17/23
19 C6* 00295 18/24
19 D1* 05267 14/24
19 D2 09740 9/22
19 E5 04198 10/24
20 C12* 00412# 16/24
20 E2* 04813 10/24
20 F6/F7 00870 11/22
20 F10 01857 13/23
21 D11(het) 09716 13/24
22 A4/A5 00471 16/24
22 A8* 00483 15/24
22 B10 01460 15/24
22 F6* 01393 13/23
22 G3 02012 16/24
24 A6 00970 9/24
24 D5* 07204 12/24
24 H3* 01162 9/23
25 C4 04301 14/24
25 C7 07624 9/24
25 H1 02256 12/23
26 A9* 08909 17/23
26 D6* 07649 10/23
26 D10 03899 8/24
26 G6/G7 07482 9/24 & 11/24
26 H2* 02194# 11/24
27 C4* 06865 9/23
27 C10* 07344 14/24
27 E6* 09576 15/23
28 D2 00487 23/48
28 E10* 00743 17/23
28 F12* 02113 4/16
29 D6(het) 07591 11/24
29 E3* 08927 13/23
30 B5 01866 15/24
30 B11 05939 41/71
30 B12/C1 06149 23/24 (likely closely linked)
30 C3* 06199 16/24
30 F5/F6 01471 14/36
30 G1(het) 06171 29/48
30 G10 (het) 07683 17/24
30 G12* 09159 11/16
31 A5 01859 15/20
31 B1 05133 13/24
31 B2 05128 8/24
31 B3/B4 05113 14/24
31 B5/B6 05079 12/23
31 C5* 05028 12/24
31 F11 03171 14/24
32 C2* 03223# 14/24
32 C3 03267 14/24
32 E3/E4 00461# 10/24
32 E8 01993 15/24
32 F10/F11 05089 14/24
32 G1* 05159 8/23
33 A6 05104 13/24
33 C10 05693 54/70
33 F5 (het) 07521 11/24
33 H11* 06059 12/21
34 E4(het) 02423 12/20
34 F9/F10 00680 13/24
35 D5* 05592 12/23
35 E6/E7 01383 11/23 & 14/24
35 E10 01450 15/24
35 H8* 01807 8/13
37 C10 05777 11/16
37 D8* 06389 17/24
37 G11* 05542 11/24
37 H2* 05580 10/24
37 H8* 06113 15/23
38 F11* 03530 11/22
39 H1 01059 29/39
39 H7* 01086 11/24
40 A3 01400 11/23
40 E12 05789 13/24
40 H1 01215 12/23
40 H9 03078 12/24
41 D3 01924 12/22
41 F5 05195 16/24
41 G3/G4 02779 15/24
42 G6* 00805 14/24
43 H5 (het) 01335 10/22
44 A10 04105 likely to be contaminated
44 F5 04442 10/24
45 D3 05683 14/24
45 D12 08131 11/23 & 10/24
45 G1 07732 7/16
46 D12* 01557 16/24
47 H11/H12 00938 24/38
48 D8* 00929 15/24
49 A3 00061 11/22
49 F4* 05803 11/23
50 B3 4549 18/22
50 C3 07095 9/24
50 G11/G12 04607 8/16
51 E3* 07135 XXX
51 F2 07180 10/17
51 F4 07187 11/24
53 A8 05127 15/24
53 C9 05271 21/24
53 F4/F5 05262 -----
54 A12 02588 9/12
54 H8 03720 11/24
56 G6 02340 very sick
61 F10 06056 7/15
62 B8 06156# 13/24
65 F6/F7 04882# 13/23
66 G1 07032 13/18
67 G11 04240 ------
67 H11* 04475 14/23
68 A4 04598 4/9
68 E11 04706 14/24
70 A11* 05665 4/12
70 C6* 04722# 11/23
70 D12* 05784 9/24
73 G5* 00084 11/24
73 H10* 06233 10/24
74 G8* 03048 14/24
75 D4 02660 16/24
75 G1* 03994 6/11
75 H12* 00590 12/23
76 B8* 09973 8/22
76 C9* 01187 17/24
76 D12 05655 14/20
76 E1 05658 10/21
76 E12 07399 10/18
76 F1 07872 8/24
76 F3 08177 14/23
76 H3* 10367 -----
78 A10 02467 11/23
78 E2/E3 06031 8/21 & 11/24
81 E11 05653 11/23
82 A8 07059 10/24
82 C7* 06491 12/21
82 F8* 06891 12/23
83 A12 03071 9/13
86 C7 08431 12/23
86 C8* 08555 8/24
88 G4* 09100 14/23
89 F12 04495 19/24
91 B12* 07190 -----
91 H4* 00966 7/13
93 H12 03697 11/15
96 D9 05299 12/24
96 E9/E10 05429 19/24
102 B9 08248 -----
103 H11/H12 10049 -----
104 F8 02017 18/24
106 H6* 09674 -----
107 D8* 03527 -----
107 H8* 04352 14/21
 
 
 
Identified cell fusion genes.
 
1 H8/H9 2784 ham-1/so WW domain
2 A3/A4 8741 ham-3 striatin
2 B10/B11 6419 mek-1 MAPKK
2B12 29A2 11376 mak-1 MAPK
2 C6/C7 2234 mik-1 MAPKKK
4 A8 6182 nrc-1 MAPKKK
4 B11/F6 4612 mek-2 MAPKK
4 B12 2393 mak-2 MAPK
6 D5/D6 07392 adv-1 trans factor
6 F10 02896 ada-3 trans factor
6 B10 04866 ada-6 trans factor
7 H9/H10 00421 snf-5 trans factor
10 B6/B7 6563 pp-2A protein phosphatase
12H6/H7 0528 ham-4 phosphopeptide binding
13 C3 7674 mob-3 phocein
14 A5 3727 ham-2 striatin/phocein binder
17 F7/F8 1069 amph-1 amphiphysin
29 A6/A7 6205 rco-1 trans factor
29 A8 2160 rac-1 G protein
40 B12/C1 0881 ham-7 GPI anchored - complemented
42 B7 0506 pkr-1 V-ATPase assembly in ER- comple.
47 E11 48 E5 2767 ham-6 membrane protein - complemented
55 H9 2811 ham-8 membrane protein - complemented
62 D8/D9 1789 ham-5 nuclear transfer of MAPK
98A1 7389 ham-9 SAM & PH domain protein
107 F3(het) 0340 pp-1 trans factor
108 C3/C4 2833 ham-10 C2 domain protein
Not in library 6842 rcm-1 trans factor
 
 
 
 
 
Individual plate listings
Plate Number FGSC #'s Strain locations
Plate 1 11201-11296 Excel
Plate 2 11297-11392 Excel
Plate 3 11393-11458 Excel
Plate 4 11459-11526 11668-11689 Excel
Plate 5 11097-11147 Excel
Plate 6 11001-11096 Excel
Plate 7 11690-11788 Excel
Plate 8 11789-11890 Excel
Plate 9 11891-11988 Excel
Plate 10 11528-11637 Excel
Plate 11 11638-11667, 11148-11200 Excel
Plate 12 11989-12086 Excel
Plate 13 12335-12433 Excel
Plate 14 12087-12184 Excel
Plate 15 12434-12530 Excel
Plate 16 12185-12284 Excel
Plate 17 12285-12334, 12531-12576 Excel
Plate 18 12581- 12680 Excel
Plate 19 15670- 15770 Excel
Plate 20 12681- 12776 Excel
Plate 21 15771- 15869 Excel
Plate 22 12777- 12874 Excel
Plate 23 15726 - 15969 Excel
Plate 24 12799 - 12968 Excel
Plate 25 15970 - 16068 Excel
Plate 26 12969 - 13070 Excel
Plate 27 13071 - 13166 Excel
Plate 28 15996, 16070 - 16170 Excel
Plate 29 Morphological Mutants Excel
Plate 30 13167-13268 Excel
Plate 31 13269-13365 Excel
Plate 32 13366-13461 Excel
Plate 33 13463-13559 Excel
Plate 34 16171-16267 Excel
Plate 35 13393-13653 Excel
Plate 36 16268-16363 Excel
Plate 37 13654 - 13751 Excel
Plate 38 16364 - 16459 Excel
Plate 39 16460 - 16555 Excel
Plate 40 13752-13847 Excel
Plate 41 13846-13941 Excel
Plate 42 16556-16652 Excel
Plate 43 16653-16752 Excel
Plate 44 16749-16844 Excel
Plate 45 13942 -14037 Excel
Plate 46 14038-14133 Excel
Plate 47 16845-16940 Excel
Plate 48 16941-17036 Excel
Plate 49 14138-14233 Excel
Plate 50 14234-14329 Excel
Plate 51 14330-14425 Excel
Plate 52 14426-14521 Excel
Plate 53 14522-14617 Excel
Plate 54 17037-17132 Excel
Plate 55 17133-17228 Excel
Plate 56 17229- 17324 Excel
Plate 57 14618-14713 Excel
Plate 58 14714- 14809 Excel
Plate 59 14810-14905 Excel
Plate 60 17325-17420 Excel
Plate 61 14906-15001 Excel
Plate 62 15002-15097 Excel
Plate 63 17421-17516 Excel
Plate 64 15098-15195 Excel
Plate 65 17517-17612 Excel
Plate 66 15196-15291 Excel
Plate 67 15292-15387 Excel
Plate 68 17613-17708 Excel
Plate 69 14134-15479 Excel
Plate 70 17709-17804 Excel
Plate 71 15480-15575 Excel
Plate 72 17805-17900 Excel
Plate 73 15576-15669, 19672-19673 Excel
Plate 74 19674-19769 Excel
Plate 75 19770-19865 Excel
Plate 76 17902-17999 Excel
Plate 77 19866-19961 Excel
Plate 78 19962-20057 Excel
Plate 79 17999-18094 Excel
Plate 80 18095-18190 Excel
Plate 81 20058-20153 Excel
Plate 82 17901-20247 Excel
Plate 83 18181-18286 Excel
Plate 84 20248-20343 Excel
Plate 85 18287-18382 Excel
Plate 86 19034-19129 Excel
Plate 87 18383-20346 Excel
Plate 88 19130-19225 Excel
Plate 89 19005-19292 Excel
Plate 90 18476-20351 Excel
Plate 91 19293-19388 Excel
Plate 92 19389-20374 Excel
Plate 93 18567-18662 Excel
Plate 94 18663-18758 Excel
Plate 95 18759-18854 Excel
Plate 96 18855-18949, 18952 Excel
Plate 97 18952-19548 Excel
Plate 98 19549-19627, 18961-18977 Excel
Plate 99 18976-19004, 20375-20441 Excel
Plate 100 19628-19671, 20442-21029 Excel
Plate 101   Excel
Plate 102   Excel
Plate 103   Excel
Plate 104   Excel
Plate 105   Excel
Plate 106   Excel
Plate 107   Excel
Plate 108   Excel
Plate 109   Excel
Plate 110   Excel

 


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3/22/11