Co-segregation data from Dr. S. Free
|
Below is a list of those wells in the KO library which contain
protoperithecia-defective isolates in which the protoperithecia-defective
phenotype does not cosegregate with the hygromycin insertion/KO mutation. An *
in the well# column means that a second isolate of the other mating type exists
in the library and the second isolate does not have the
protoperithecia-defective phenotype. A # in the NCU# column means that our PCR
assay suggests that the hygromycin mutation does not disrupt the indicated gene.
The information in the cosegregation data column is given as the number of
parental type progeny/total progeny. An XXX in the coseg. data column indicates
that because the second isolate for the deletion strain was not affected in
properithcia development, it was clear that the mutant phenotype was not due to
the deletion and the strain was not tested. An ---- in the coseg data column
indicates that the ratio of parental type progeny/total progeny was not recorded
but that the strain was recorded as not showing co-segregation between the
protoperithecia defective phenotype and hygromycin resistance. Plate # well# NCU# coseg. data 1 A8* 01312 XXX 1 D11* 06650 ----- 1 F4 09866 not recovered for second screen 1 G10* 00157 11/21 & 14/24 1 H1 00810 10/18 2 A5 07621 8/24 2 B9* 4096 ----- 2 D9 07622 14/24 2 E11 3043 not recovered for second screen 3 A5 00090 13/24 but coseg morph phenotype 3 B3* 06366 11/23 3 D8 07024 contaminated well 3 D9 00019 16/24 4 E3 00117 17/23 4 H3/H4 07817 18/24 5 D4 04561 8/24 5 A12(ada-2) 02017 contaminated well (proto+) 6 E5(ada-5) 03931 14/23 6 E10(ada-4) 03320 12/24 6 F2(ada-7) 06068 12/24 6 G5* 03686 12/24 7 A1/A2 03800 14/24 7 A3/A4 02692 11/23 7 H4 03875 14/22 but coseg growth phenotype 8 D3* 02211 15/24 8 G7 02734 9/24 9 C10 07361 16/23 9 E10* 08739 14/24 10 C11 04145 15/24 10 H6* 05196 10/24 11 D3* 02795 16/24 11 G4* 06605 11/24 12 A8/A9 04350 10/24 12 A11* 00481 15/23 12 G1* 07381 10/24 13 B4* 09611 11/24 13 B12* 01143 11/24 13 E8* 00284 18/24 13 E10* 03850 12/24 13 E11 03854 11/22 13 F3* 03862 8/24 13 F12/G1 04583 15/24 & 16/24 13 H11 02534 9/24 15 A4 04078 10/23 15 B2 09432 12/22 15 B8/B9 08301 ------ 16 B5 00159 not recovered for second screen 16 E6* 01062 13/24 17 C4* 06710 16/24 17 F2 05157 12/24 17 F9/F10 02354 13/24 but coseg growth phenotype 24/24 17 G1* 06112 17/23 19 C6* 00295 18/24 19 D1* 05267 14/24 19 D2 09740 9/22 19 E5 04198 10/24 20 C12* 00412# 16/24 20 E2* 04813 10/24 20 F6/F7 00870 11/22 20 F10 01857 13/23 21 D11(het) 09716 13/24 22 A4/A5 00471 16/24 22 A8* 00483 15/24 22 B10 01460 15/24 22 F6* 01393 13/23 22 G3 02012 16/24 24 A6 00970 9/24 24 D5* 07204 12/24 24 H3* 01162 9/23 25 C4 04301 14/24 25 C7 07624 9/24 25 H1 02256 12/23 26 A9* 08909 17/23 26 D6* 07649 10/23 26 D10 03899 8/24 26 G6/G7 07482 9/24 & 11/24 26 H2* 02194# 11/24 27 C4* 06865 9/23 27 C10* 07344 14/24 27 E6* 09576 15/23 28 D2 00487 23/48 28 E10* 00743 17/23 28 F12* 02113 4/16 29 D6(het) 07591 11/24 29 E3* 08927 13/23 30 B5 01866 15/24 30 B11 05939 41/71 30 B12/C1 06149 23/24 (likely closely linked) 30 C3* 06199 16/24 30 F5/F6 01471 14/36 30 G1(het) 06171 29/48 30 G10 (het) 07683 17/24 30 G12* 09159 11/16 31 A5 01859 15/20 31 B1 05133 13/24 31 B2 05128 8/24 31 B3/B4 05113 14/24 31 B5/B6 05079 12/23 31 C5* 05028 12/24 31 F11 03171 14/24 32 C2* 03223# 14/24 32 C3 03267 14/24 32 E3/E4 00461# 10/24 32 E8 01993 15/24 32 F10/F11 05089 14/24 32 G1* 05159 8/23 33 A6 05104 13/24 33 C10 05693 54/70 33 F5 (het) 07521 11/24 33 H11* 06059 12/21 34 E4(het) 02423 12/20 34 F9/F10 00680 13/24 35 D5* 05592 12/23 35 E6/E7 01383 11/23 & 14/24 35 E10 01450 15/24 35 H8* 01807 8/13 37 C10 05777 11/16 37 D8* 06389 17/24 37 G11* 05542 11/24 37 H2* 05580 10/24 37 H8* 06113 15/23 38 F11* 03530 11/22 39 H1 01059 29/39 39 H7* 01086 11/24 40 A3 01400 11/23 40 E12 05789 13/24 40 H1 01215 12/23 40 H9 03078 12/24 41 D3 01924 12/22 41 F5 05195 16/24 41 G3/G4 02779 15/24 42 G6* 00805 14/24 43 H5 (het) 01335 10/22 44 A10 04105 likely to be contaminated 44 F5 04442 10/24 45 D3 05683 14/24 45 D12 08131 11/23 & 10/24 45 G1 07732 7/16 46 D12* 01557 16/24 47 H11/H12 00938 24/38 48 D8* 00929 15/24 49 A3 00061 11/22 49 F4* 05803 11/23 50 B3 4549 18/22 50 C3 07095 9/24 50 G11/G12 04607 8/16 51 E3* 07135 XXX 51 F2 07180 10/17 51 F4 07187 11/24 53 A8 05127 15/24 53 C9 05271 21/24 53 F4/F5 05262 ----- 54 A12 02588 9/12 54 H8 03720 11/24 56 G6 02340 very sick 61 F10 06056 7/15 62 B8 06156# 13/24 65 F6/F7 04882# 13/23 66 G1 07032 13/18 67 G11 04240 ------ 67 H11* 04475 14/23 68 A4 04598 4/9 68 E11 04706 14/24 70 A11* 05665 4/12 70 C6* 04722# 11/23 70 D12* 05784 9/24 73 G5* 00084 11/24 73 H10* 06233 10/24 74 G8* 03048 14/24 75 D4 02660 16/24 75 G1* 03994 6/11 75 H12* 00590 12/23 76 B8* 09973 8/22 76 C9* 01187 17/24 76 D12 05655 14/20 76 E1 05658 10/21 76 E12 07399 10/18 76 F1 07872 8/24 76 F3 08177 14/23 76 H3* 10367 ----- 78 A10 02467 11/23 78 E2/E3 06031 8/21 & 11/24 81 E11 05653 11/23 82 A8 07059 10/24 82 C7* 06491 12/21 82 F8* 06891 12/23 83 A12 03071 9/13 86 C7 08431 12/23 86 C8* 08555 8/24 88 G4* 09100 14/23 89 F12 04495 19/24 91 B12* 07190 ----- 91 H4* 00966 7/13 93 H12 03697 11/15 96 D9 05299 12/24 96 E9/E10 05429 19/24 102 B9 08248 ----- 103 H11/H12 10049 ----- 104 F8 02017 18/24 106 H6* 09674 ----- 107 D8* 03527 ----- 107 H8* 04352 14/21 Identified cell fusion genes. 1 H8/H9 2784 ham-1/so WW domain 2 A3/A4 8741 ham-3 striatin 2 B10/B11 6419 mek-1 MAPKK 2B12 29A2 11376 mak-1 MAPK 2 C6/C7 2234 mik-1 MAPKKK 4 A8 6182 nrc-1 MAPKKK 4 B11/F6 4612 mek-2 MAPKK 4 B12 2393 mak-2 MAPK 6 D5/D6 07392 adv-1 trans factor 6 F10 02896 ada-3 trans factor 6 B10 04866 ada-6 trans factor 7 H9/H10 00421 snf-5 trans factor 10 B6/B7 6563 pp-2A protein phosphatase 12H6/H7 0528 ham-4 phosphopeptide binding 13 C3 7674 mob-3 phocein 14 A5 3727 ham-2 striatin/phocein binder 17 F7/F8 1069 amph-1 amphiphysin 29 A6/A7 6205 rco-1 trans factor 29 A8 2160 rac-1 G protein 40 B12/C1 0881 ham-7 GPI anchored - complemented 42 B7 0506 pkr-1 V-ATPase assembly in ER- comple. 47 E11 48 E5 2767 ham-6 membrane protein - complemented 55 H9 2811 ham-8 membrane protein - complemented 62 D8/D9 1789 ham-5 nuclear transfer of MAPK 98A1 7389 ham-9 SAM & PH domain protein 107 F3(het) 0340 pp-1 trans factor 108 C3/C4 2833 ham-10 C2 domain protein Not in library 6842 rcm-1 trans factor |
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| Individual plate listings | ||
| Plate Number | FGSC #'s | Strain locations |
| Plate 1 | 11201-11296 | Excel |
| Plate 2 | 11297-11392 | Excel |
| Plate 3 | 11393-11458 | Excel |
| Plate 4 | 11459-11526 11668-11689 | Excel |
| Plate 5 | 11097-11147 | Excel |
| Plate 6 | 11001-11096 | Excel |
| Plate 7 | 11690-11788 | Excel |
| Plate 8 | 11789-11890 | Excel |
| Plate 9 | 11891-11988 | Excel |
| Plate 10 | 11528-11637 | Excel |
| Plate 11 | 11638-11667, 11148-11200 | Excel |
| Plate 12 | 11989-12086 | Excel |
| Plate 13 | 12335-12433 | Excel |
| Plate 14 | 12087-12184 | Excel |
| Plate 15 | 12434-12530 | Excel |
| Plate 16 | 12185-12284 | Excel |
| Plate 17 | 12285-12334, 12531-12576 | Excel |
| Plate 18 | 12581- 12680 | Excel |
| Plate 19 | 15670- 15770 | Excel |
| Plate 20 | 12681- 12776 | Excel |
| Plate 21 | 15771- 15869 | Excel |
| Plate 22 | 12777- 12874 | Excel |
| Plate 23 | 15726 - 15969 | Excel |
| Plate 24 | 12799 - 12968 | Excel |
| Plate 25 | 15970 - 16068 | Excel |
| Plate 26 | 12969 - 13070 | Excel |
| Plate 27 | 13071 - 13166 | Excel |
| Plate 28 | 15996, 16070 - 16170 | Excel |
| Plate 29 | Morphological Mutants | Excel |
| Plate 30 | 13167-13268 | Excel |
| Plate 31 | 13269-13365 | Excel |
| Plate 32 | 13366-13461 | Excel |
| Plate 33 | 13463-13559 | Excel |
| Plate 34 | 16171-16267 | Excel |
| Plate 35 | 13393-13653 | Excel |
| Plate 36 | 16268-16363 | Excel |
| Plate 37 | 13654 - 13751 | Excel |
| Plate 38 | 16364 - 16459 | Excel |
| Plate 39 | 16460 - 16555 | Excel |
| Plate 40 | 13752-13847 | Excel |
| Plate 41 | 13846-13941 | Excel |
| Plate 42 | 16556-16652 | Excel |
| Plate 43 | 16653-16752 | Excel |
| Plate 44 | 16749-16844 | Excel |
| Plate 45 | 13942 -14037 | Excel |
| Plate 46 | 14038-14133 | Excel |
| Plate 47 | 16845-16940 | Excel |
| Plate 48 | 16941-17036 | Excel |
| Plate 49 | 14138-14233 | Excel |
| Plate 50 | 14234-14329 | Excel |
| Plate 51 | 14330-14425 | Excel |
| Plate 52 | 14426-14521 | Excel |
| Plate 53 | 14522-14617 | Excel |
| Plate 54 | 17037-17132 | Excel |
| Plate 55 | 17133-17228 | Excel |
| Plate 56 | 17229- 17324 | Excel |
| Plate 57 | 14618-14713 | Excel |
| Plate 58 | 14714- 14809 | Excel |
| Plate 59 | 14810-14905 | Excel |
| Plate 60 | 17325-17420 | Excel |
| Plate 61 | 14906-15001 | Excel |
| Plate 62 | 15002-15097 | Excel |
| Plate 63 | 17421-17516 | Excel |
| Plate 64 | 15098-15195 | Excel |
| Plate 65 | 17517-17612 | Excel |
| Plate 66 | 15196-15291 | Excel |
| Plate 67 | 15292-15387 | Excel |
| Plate 68 | 17613-17708 | Excel |
| Plate 69 | 14134-15479 | Excel |
| Plate 70 | 17709-17804 | Excel |
| Plate 71 | 15480-15575 | Excel |
| Plate 72 | 17805-17900 | Excel |
| Plate 73 | 15576-15669, 19672-19673 | Excel |
| Plate 74 | 19674-19769 | Excel |
| Plate 75 | 19770-19865 | Excel |
| Plate 76 | 17902-17999 | Excel |
| Plate 77 | 19866-19961 | Excel |
| Plate 78 | 19962-20057 | Excel |
| Plate 79 | 17999-18094 | Excel |
| Plate 80 | 18095-18190 | Excel |
| Plate 81 | 20058-20153 | Excel |
| Plate 82 | 17901-20247 | Excel |
| Plate 83 | 18181-18286 | Excel |
| Plate 84 | 20248-20343 | Excel |
| Plate 85 | 18287-18382 | Excel |
| Plate 86 | 19034-19129 | Excel |
| Plate 87 | 18383-20346 | Excel |
| Plate 88 | 19130-19225 | Excel |
| Plate 89 | 19005-19292 | Excel |
| Plate 90 | 18476-20351 | Excel |
| Plate 91 | 19293-19388 | Excel |
| Plate 92 | 19389-20374 | Excel |
| Plate 93 | 18567-18662 | Excel |
| Plate 94 | 18663-18758 | Excel |
| Plate 95 | 18759-18854 | Excel |
| Plate 96 | 18855-18949, 18952 | Excel |
| Plate 97 | 18952-19548 | Excel |
| Plate 98 | 19549-19627, 18961-18977 | Excel |
| Plate 99 | 18976-19004, 20375-20441 | Excel |
| Plate 100 | 19628-19671, 20442-21029 | Excel |
| Plate 101 | Excel | |
| Plate 102 | Excel | |
| Plate 103 | Excel | |
| Plate 104 | Excel | |
| Plate 105 | Excel | |
| Plate 106 | Excel | |
| Plate 107 | Excel | |
| Plate 108 | Excel | |
| Plate 109 | Excel | |
| Plate 110 | Excel | |
3/22/11