FGSC #9064

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Reporting Genes: het-c^OR pyr-4; vib-1

Species: crassa

Allele: OR - ; (1)

Alternate Strain Number: X80-32

Depositor: NLG

Mating Type: A

ref1: Xiang and Glass 2002 Genetics 162:89-101, https://doi.org/10.1093/genetics/162.1.89

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group
pyr-4	IIL. Right of het-c (1%), T(P2869), and cys-3 (18 to 21%). Left of ro-3 (1 to 2%) (721, 816, PB). (812). Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate decarboxylase (133, 134, 841) (Fig. 20). Fertile crosses homozygous for pyr-4 can be made by using very high levels of uridine (15 to 20 mg/ml) (O.M. Mylyk, personal communication).	IIL
het-c^OR	Two alleles found in lab strains were originally called het-C and het-c. Following the discovery and demonstration of a third allele, het-c^PA, the symbol het-C was changed to het-c^OR and the symbol het-c was changed to het-c^EM. Three specificity types have been recognized and shown to be polymorphic in nature. These are designated het-c^OR, het-c^GR, and het-c^PA.
vib-1	Mutations in the vib-1 locus suppress growth inhibition and conidiation repression by genetic differences at het-c and mat and reduce HCD. The vib-1 locus encodes a nuclear localized putative transcriptional regulator. HET-C heterocomplex formation occurs in the vib-1 mutant, indicating that VIB-1 functions downstream of HET-C heterocomplex formation and may mediate some of the phenotypic responses to heterokaryon incompatibility at the transcriptional level.