FGSC #9061

Reporting Genes: pyr-4; ham-2

Species: crassa

Allele: RIP -

Alternate Strain Number: CRL-10

Depositor: NLG

Linkage Group: IIL; V

Mating Type: A

Species Number: 10

ref1: Xiang et al 2002 Genetics 160:169-180, https://doi.org/10.1093/genetics/160.1.169

Genes

Locus	Cultural Requirements	Link Group	Type
pyr-4	IIL. Right of het-c (1%), T(P2869), and cys-3 (18 to 21%). Left of ro-3 (1 to 2%) (721, 816, PB). (812). Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate decarboxylase (133, 134, 841) (Fig. 20). Fertile crosses homozygous for pyr-4 can be made by using very high levels of uridine (15 to 20 mg/ml) (O.M. Mylyk, personal communication).	IIL	B
ham-2 (rip)	A hyphal fusion mutant gene. The mutant has a greatly reduced ability to fuse with itself or with wild type strains. The ability of the ham-2 mutant to fuse is reduced by a factor of a thousand fold when compared to wild type. Other phenotypes of the ham-2 mutant strains include a reduced growth rate, short aerial hyphae and female sterility.		B

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