FGSC #113

Reporting Genes: trp-4 pan-1

Species: crassa

Allele: Y2198 5531

Alternate Strain Number: 4026

Depositor: DDP

Linkage Group: IVR IVR

Mating Type: a

Species Number: 10

ref1: Maling 1959 Genetics 44:1215-1220, https://www.genetics.org/content/44/6/1215.long

Genes

Locus	Cultural Requirements	Link Group	Type
trp-4	IVR. Between his-5 (3 to 7%) and leu-2 (1 to 2%) (633, 991). (47). Uses tryptophan or indole (750). Deficient in anthranilate phosphoribosyl transferase (1126) (Fig. 11). Scorable by blue fluorescence (anthranilate) in medium under long-wave UV after 2 to 5 days of growth on minimal medium plus indole (10 µg /ml), 34°C. Initial stocks of the first trp-4 mutant were inhibited by suboptimal concentrations of tryptophan (750), but derivatives have been obtained that are free of this problem (909).	IVR	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B

Neurospora Crassa Wikipedia