FGSC #8914

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Reporting Genes: mepR(10) his-3; inl^[(SadI-BglII)::lambda A]

Species: crassa

Allele: his-3 (1-234-723); inl (SacI-BGlll)

Alternate Strain Number: RPNCr3A

Depositor: RJP used by DJE

Mating Type: A

Species Number: 10

ref1: Pratt and Aramayo 2002 FG&B 37:56-71, https://doi.org/10.1016/S1087-1845(02)00032-4

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B
inl(SadI-BglII)::lambdaA			B
mep(10)	Not mapped. Segregates 1:1. Resistant to 6-methylpurine. Adenine phosphoribosyltransferase activity is negligible in vitro. Adenine uptake is normal. Resistance may result from inability to convert the analog to nucleotide form. Has low hypoxanthine phosphoribosyltransferase activity, as do the mutants mep(3) and ad-8, q.v. Selected on 1 mM 6-methylpurine-sorbose medium. Not tested for allelism with mep(3). Called Mepr-10, but not indicated to be dominant. (785) Called mep-2 by Barratt and Ogata (51).		B