FGSC #8609

Reporting Genes: Tad hyg^r

Species: crassa

Alternate Strain Number: T430a (UK 97-5-101)

Depositor: JAK

Mating Type: a

Species Number: 10

ref1: Anderson et al 2001 Fungal Genetics and Biology 33:49-57, https://doi.org/10.1006/fgbi.2001.1267

ref2: Bhat et al 2003 Genetics 164:947-961, https://doi.org/10.1093/genetics/164.3.947

Genes

Locus	Cultural Requirements	Link Group	Type
hyg^r	hygromycin phosphotransferase gene construct		B
Tad	A LINE-like retrotransposon present as an active element in a strain from the Ivory Coast and discovered when the am gene was inactivated by insertion of Tad. Active Tad elements can be introduced into laboratory strains by crosses, heterokaryons, or transformation. In heterokaryons, an active Tad is transferred readily to nuclei that previously lacked it; if Tad in the donor nucleus contained an intron, the intron is spliced out prior to transfer. When a strain that previously lacked an active Tad acquires it, the element remains active during vegetative growth and propagation. It is inactivated by RIP when it goes through a cross, however. Lab strains and strains representing seven heterothallic and homothallic Neurospora species contain inactive Tad-like sequences, and these appear to have undergone RIP.		B

Neurospora Crassa Wikipedia