FGSC #8594

Mutant Type

Genus: N

reporting_genes: his-3; dim-2::hph

species: Neurospora crassa

allele: 1-234-723; EK1

stock: EUS N1877

glasgow:

mutagen:

Depositor: EUS

Link Group: IR, VIIR

MT: a

Species No: 10

gene_back: M

oppmt:

trans:

ref1: Kouzminova and Selker 2001 EMBO J 20:4309-4323, https://doi.org/10.1093/emboj/20.15.4309

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-8594

Locus	Cultural Requirements	Link Group	Type
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B
dim-2::hph			B

Neurospora Crassa Wikipedia