FGSC #32

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Reporting Genes: nit-1

Species: crassa

Allele: 34547

Alternate Strain Number: 1053

Mutagen: UV

Depositor: DDP

Linkage Group: IR

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 54

ref1: Perkins 1959 Genetics 44:1185-1208, https://www.genetics.org/content/44/6/1185.long

ref2: https://doi.org/10.1016/s0065-2660(08)60127-3

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nit-1	IR. Right of Tp(T54M94) and ad-9 (3 to 15%). Left of cyh-1(6%) (466, 496, 816). (482)Cannot use nitrate or hypoxanthine as a nitrogen source, but uses nitrite, ammonia, or amino acids (1000). Does not prevent formation or nitrate reductase apoprotein (999), but lacks the molybdenum-containing cofactor common to nitrate reductase and xanthine dehydrogenase (591, 741) (Fig. 19 and 24). The nitrate reductase in nit-1 extracts does not catalyze the complete electron transport sequence from NADPH to N03 but does catalyze the initial part of this sequence if a suitable electron acceptor (e.g., cytochrome c) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. See references 226, 999, and 1000 for regulation.	IR	B