FGSC #8386

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Reporting Genes: spe-1; aga

Species: crassa

Allele: LV10; UM906

Alternate Strain Number: 1894-53a

Mutagen: UV

Depositor: RHD

Linkage Group: VR; VIIR

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Davis et al 1987 Mol Cell Biol 7:1122-1128, https://doi.org/10.1128/mcb.7.3.1122-1128.1987

ref2: Pitkin and Davis 1990 Arch Biochem Biophys 278:386-391, https://doi.org/10.1016/0003-9861(90)90275-4

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
aga	VIIR. Between wc-1 (2%) and arg-10 (24 to 27%) (240, 697). Presumed structural gene for arginase (240, 697) (see Fig. 10). Unable to form ornithine from arginine; arginine is thus unable to satisfy the proline requirement of pro-3 in a pro-3 aga double mutant. Prototrophic single mutants develop polyamine requirement in the presence of arginine. This is due to feedback inhibition of ornithine biosynthesis by arginine, combined with a catabolic block in ornithine formation from arginine (240). Siderophore production is severely reduced in the absence of ornithine in the triple mutant aga arg-5 ota, which has been used to study iron transport (1146, 1147) and to obtain mutants defective in siderophore uptake (G.W. Charlang and N.P. Williams, personal communication); see sit.	VIIR	B
spe-1	VR. Linked to cyh-2 (2%); left of inl (12%) (657, PB). Uses putrescine, spermidine, or spermine. Affects ornithine decarboxylase (L-ornithine carboxy-lyase) (Fig. 10). Does not suppress pro-4 (657). Excretes yellow pigment into synthetic cross medium (PB). Meiosis normal in homozygous crosses (on 50 µg of spermidine per ml), which produce mostly white and a few viable black ascospores (N.B. Raju, personal communication; R. H. Davis, personal communication). Formerly called put-1.	VR	B