FGSC #8379

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Reporting Genes: lacc nic-3

Species: crassa

Allele: HR6; Y31881

Alternate Strain Number: HR29-19a

Depositor: HI

Linkage Group: VII

Mating Type: a

Species Number: 10

ref1: Tamaru et al 1994 MGG 243:548-554, https://doi.org/10.1007/bf00284203

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nic-3	VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.	VIIL	B
lacc	VIIL. Between nic-3 (14%) and thi-3 (7%). Laccase structural gene (benzenediol:oxygen oxidoreductase, urishol oxidase), extracellular. Alleles from wild-type strains TS and OR sequenced. The laccase polypeptide is processed posttranslationally at both its N- and C-termini. lacc mutants do not show a detectable phenotype except that laccase is not produced. Gene expression is induced by inhibitors of RNA or protein synthesis or by certain aromatic molecules. The secreted enzyme forms a blue halo of oxidezed o-tolidine around colonies in test plates. Screening for mutants that overproduced laccase led to the ident. of lah-1. Screening for additional mutants that did not allow the overexpression of laccase in the lah-1 background established that functional cpc-1 was required for lacc induction. Called lni-2.	VIIL	B