FGSC #9

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Reporting Genes: for

Species: crassa

Allele: C24

Alternate Strain Number: 7066

Mutagen: UV

Depositor: DDP

Linkage Group: VIIR

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 3850

ref1: Harrold and Fling, 1952. JBC 194:399-406, https://doi.org/10.1016/S0021-9258(18)55891-9

ref2: Lein, J. et al. 1948. PNAS 34:435-42, https://doi.org/10.1073/pnas.34.9.435, https://thesis.library.caltech.edu/1449/1/Dubes_gr_1953.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
for	VIIR. Right of wc-1 (5%) and frq (3%). Left of dr (3%) (812, 819; J. F. Feldman, personal communication).Requires formate or formaldehyde. Growth aided slightly if glycine, histidine, or choline is added to formate (446). Will also grow on a mixture of methionine and adenine and suboptimally on adenine alone (446). Lacks cytosolic (but not mitochondrial) serine hydroxymethyl- transferase (Fig. 17) (124, 210). Has increased formyl tetrahydrofolate synthetase, methylene tetrahydrofolate dehydrogenase, isocitrate lyase, and glyoxalate aminotransferase activities (209, 210). Use 0.3 mg of formate per ml. Can be autoclaved.	VIIR	B