FGSC A1010

Reporting Genes: A flavus White leu aflR pyrG

Species: flavus

Alternate Strain Number: 656-2

Depositor: GA Payne

Species Number: 6

ref1: Appl. Environ. Microbiol. October 1997 vol. 63 no. 10 3995-4000, https://doi.org/10.1128/AEM.63.10.3995-4000.1997

Genes

Locus	Cultural Requirements	Link Group	Type
pyrG	Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil	IL	A
leu	For biosynthetic pathway, see Fig. 15. Leucine mutants have been used extensively for studies of regulation (see references 427 and 833). Leucine mutants acquire suppressors when grown on Difco agar-sorbose medium. The suppressors are leaky auxotrophs blocked at various steps in sulfur metabolism; apparently these blocks allow more efficient use of the traces of leucine in the agar (425; S. R. Gross, personal communication). Most aliphatic and aromatic amino acids can inhibit growth of leucine mutants at appropriate concentrations, probably because of competition for a common uptake system (S. R. Gross, personal communication). For regulation, see individual loci; reviewed in references 427 and 642.		B
aflR	Sterigmatocystin biosynthesis regulation	IV	A

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