FGSC #8269

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Reporting Genes: tre; mcm; pab-1

Species: crassa

Allele: 39-3; RM124-2; 1633

Alternate Strain Number: A103-39

Depositor: RM

Linkage Group: IR; IIL; VR

Mating Type: a

Species Number: 10

Genetic Background: M

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mcm	IIL. Linked to ro-7 (1%). When macroconidia from agar-grown mycelial cultures are germinated in liquid shake culture at 22°C, the germlings produce uninucleate microconidia within 24 hr, reaching concentrations of 10^7/ml at 72 hr. At 30°C, multinucleate arthroconidia and few or no micrconidia are produced. The morphology of surface-grown cultures is normal wild type.	IIL	B
pab-1	VR. Between inl (1 to 10%) and met-3 (1 to 2%) (3629 1036). (47) Requires p-aminobenzoic acid (1057) (Fig. 11). Apparently cannot use folate (mono- or triglutamate) (1179).	VR	B
tre	IR. Between met-6 (7%) and al-2 (20 to 37%); near mig(< < 1%) (1045, 1176). Shown between met-6 and ad-9 (7%) in reference 466, with only two-point data given. Unable to use trehalose as carbon source. Lacks trehalase activity. (Also, the invertase level is reduced 50% and the amylase level is increased.) In (tre + tre+) heterokaryons, the trehalase level is reduced to 10% of the wildtype level, and in tre/tre+ duplications the level is reduced to 1% of the wild-type level. tre codes for trehalase inhibitor, probably a protein (1045). For a possibly related regulatory mutation, see reference 663. For putative structural gene, see mig.	IR	B