FGSC #8268

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Reporting Genes: nic-1 tre; mcm

Species: crassa

Allele: 39113 39-3; RM124-2

Alternate Strain Number: RM146-9

Depositor: RM

Linkage Group: IR R;IIL

Mating Type: a

Species Number: 10

Genetic Background: M

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nic-1	IR. Right of ace-3 (<1%), lys-1 (1%), and In(OY323). Left of os-1(10 to 29%) (2, 57, 131, 578, 789, 816, 907). (482) Uses nicotinic acid or nicotinamide, but not precursors (97, 100) (Fig. 18). Accumulates quinolinic acid (100). Used to study intralocus recombination (907). Called the q locus.	IR	B
tre	IR. Between met-6 (7%) and al-2 (20 to 37%); near mig(< < 1%) (1045, 1176). Shown between met-6 and ad-9 (7%) in reference 466, with only two-point data given. Unable to use trehalose as carbon source. Lacks trehalase activity. (Also, the invertase level is reduced 50% and the amylase level is increased.) In (tre + tre+) heterokaryons, the trehalase level is reduced to 10% of the wildtype level, and in tre/tre+ duplications the level is reduced to 1% of the wild-type level. tre codes for trehalase inhibitor, probably a protein (1045). For a possibly related regulatory mutation, see reference 663. For putative structural gene, see mig.	IR	B
mcm	IIL. Linked to ro-7 (1%). When macroconidia from agar-grown mycelial cultures are germinated in liquid shake culture at 22°C, the germlings produce uninucleate microconidia within 24 hr, reaching concentrations of 10^7/ml at 72 hr. At 30°C, multinucleate arthroconidia and few or no micrconidia are produced. The morphology of surface-grown cultures is normal wild type.	IIL	B