FGSC #8265

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Reporting Genes: pt

Species: crassa

Allele: P4418

Alternate Strain Number: 4285

Mutagen: RIP

Depositor: DDP

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Perkins et al 1997 Genetics 147:125-136, https://doi.org/10.1093/genetics/147.1.125

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pt	IVR. Right of T(S1229) and pdx-1 (2%). Left of col-4(2%) (40, 55, 808). (201) Original S4342 strain contained linked but separable insertional translocation T(S4342) (808), the presence of which should not change conclusions regarding gene order given in reference 40. Requires phenylalanine plus tyrosine (201). Lacks chorismate mutase (40, 316) (Fig. 11). Evidently the structural gene; strains carrying allele NS1 have thermolabile chorismate mutase (D.E.A. Catcheside, personal communication). NS1 strains are temperature sensitive, growing on minimal medium at 25 C, where they are readily scorable by blue fluorescence under long-wave UV and by browning of medium of aging cultures (1035). Inhibited by complex complete medium.	IVR	B