FGSC #8261

Reporting Genes: chol-2

Species: crassa

Allele: P3335

Alternate Strain Number: 6162

Mutagen: RIP

Depositor: DDP

Linkage Group: VIL

Mating Type: A

Genetic Background: M

Opposite Mating Type: 8262

ref1: Perkins et al 1997 Genetics 147:125-136, https://doi.org/10.1093/genetics/147.1.125

Genes

Locus	Cultural Requirements	Link Group
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL

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