FGSC #8255

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Reporting Genes: psi-1 pdx-1

Species: crassa

Allele: 34C, 37803

Alternate Strain Number: 4281

Depositor: DDP

Linkage Group: IVR,R

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 8256

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B
psi-1	IVR. Right of the centromere (D.R. Stadler, A.M. Towe, and M. Loo, cited in reference 619). Left of T(ALS159) and pyr-1 (4%) (808, PB). Conidial germination and hyphal growth inhibited at 35°C but normal (or nearly so) at 20°C. Protein synthesis reduced after shift to restrictive temperature. Recessive in heterokaryons. (619) Scored as an irreparable heat- sensitive unmutant (see un).	IVR	B