FGSC #8131

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Reporting Genes: chol-1

Species: crassa

Allele: Y3261

Mutagen: M

Depositor: FGSC

Linkage Group: IVR

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 1116

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-1	IVR. Linked to ad-6 (1%). probably to the right (633). (482) Requires choline (470). Also uses mono- or dimethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidylethanolamine-N-methyl transferase (222, 924). Abnormal colonial morphology on limiting choline (222). Colonies from single conidia on minimal agar medium resemble inhibited A/a duplications, with swollen hyphae and darkening in the presence of phenylalanine plus tyrosine (D.D. Perkins, unpublished data). Abnormal phospholipid composition on limited concentrations of supplement (501). Best scored late on minimal medium. Grows slightly and then stops (D.D. Perkins, unpublished data). Used to study inhibition of cytochrome-mediated respiration and of conidiation when lecithin is depleted by choline starvation (534, 535). Initial allele called 34486.	IVR	B