FGSC A965

Reporting Genes: cspA1; olvA1; bioB2; leu A1; niaD2

Species: niger

Alternate Strain Number: EK140

Depositor: EK

Linkage Group: ;I;III;IV;VIII

Species Number: 2

Genetic Background: 016

ref1: https://doi.org/10.1007/BF00259411; https://doi.org/10.4148/1941-4765.1575

ref2: https://doi.org/10.1007/BF00167138; https://doi.org/10.1007/BF00411084

Genes

Locus	Cultural Requirements	Link Group	Type
olvA1			A
niaD2	Cannot use nitrate as a nitrogen source, should be resistant to chlorate, unable to use glutamate as an N source	VIIIR	A
leu A1	requires leucine		A
leu	For biosynthetic pathway, see Fig. 15. Leucine mutants have been used extensively for studies of regulation (see references 427 and 833). Leucine mutants acquire suppressors when grown on Difco agar-sorbose medium. The suppressors are leaky auxotrophs blocked at various steps in sulfur metabolism; apparently these blocks allow more efficient use of the traces of leucine in the agar (425; S. R. Gross, personal communication). Most aliphatic and aromatic amino acids can inhibit growth of leucine mutants at appropriate concentrations, probably because of competition for a common uptake system (S. R. Gross, personal communication). For regulation, see individual loci; reviewed in references 427 and 642.		B
bioB2	requires biotin	I	A
cspA1			A

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