FGSC #7939

Reporting Genes: cyt-1 al-2

Species: crassa

Allele: C115 15300

Alternate Strain Number: AGG-70

Depositor: HB

Linkage Group: IL

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 7938

ref1: Mitchell et al. 1953. PNAS 39:606-613, https://doi.org/10.1073/pnas.39.7.606

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
cyt-1	IL. Between leu-3 (5 to 8%) (583) and T(OY321) (D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (694) Deficient in cytochromes aa3 and b. Very slow growth. Female sterile (87, 694). Scoring aided by slower growth on complete medium relative to that on minimal, presumably owing to inhibition by yeast extract (694, 816). cyt-U-9 is linked and may be allelic with the original cyt-1 mutation C115 (87; H. Bertrand, personal communication).	IL	B

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