FGSC #7594

Reporting Genes: nic-1 al-2;cya-3

Species: crassa

Allele: 3416 15300 cya-3-16

Alternate Strain Number: NCN92

Mutagen: UV,NG

Depositor: FEN

Linkage Group: IR IR;VI

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Bertrand et al. 1977 MGG 153:247-257, https://doi.org/10.1007/bf00431590

Genes

Locus	Cultural Requirements	Link Group	Type
nic-1	IR. Right of ace-3 (<1%), lys-1 (1%), and In(OY323). Left of os-1(10 to 29%) (2, 57, 131, 578, 789, 816, 907). (482) Uses nicotinic acid or nicotinamide, but not precursors (97, 100) (Fig. 18). Accumulates quinolinic acid (100). Used to study intralocus recombination (907). Called the q locus.	IR	B
cya-3	VIL. Between chol-2 (10%) and cyt-2 (10%) (87). Deficient in cytochrome aa3. Cannot reduce tetrazolium. Slow growth (87). aa3 deficiency suppressed by antimycin A (84). Spectrum (84).	VIL	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B

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