FGSC #7546

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Reporting Genes: fl ff-1

Species: crassa

Allele: P JC744

Alternate Strain Number: CSN6

Depositor: SC

Linkage Group: IIR IIR

Mating Type: a

Species Number: 10

Opposite Mating Type: 7545

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ff-1	IIR. Between aro-1 (5%) and un-20 (4%) (1052). (1053)Female sterile with no protoperithecia (1052, 1053). Enhanced glycerol utilization; aconidial on liquid glycerol medium. Good conidiation, but reduced aerial hyphae and heavy surface growth on sucrose minimal slants. Growth on glycerol reduces levels of both pyruvate dehydrogenase and dihydrolipoyl transacetylase (211). Effect of carbon source studied (1078). Conveniently scored by failure to produce protoperithecia on small slants of synthetic cross medium (7 days, 25 C). Allele T30 was originally recognized as specifying female sterility and was called ff-1 (1052, 1053). Allele JC744 was first characterized by glycerol utilization and called glp-3 (211).	IIR	B
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B