FGSC #7509

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Reporting Genes: wc-1 nt sk

Species: crassa

Allele: P829 65001 P1718

Depositor: FGSC

Linkage Group: VIIR R R

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 6660

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nt	VIIR. Between arg-10 (2 to 12%) and sk (7 to 18%) (789). (874) Uses nicotinic acid. May respond also to tryptophan, phenylalanine, tyrosine, quinic acid, and precursors of nicotinic acid or tryptophan, or both, depending on genetic background (448, 760). Best supplemented with nicotinamide and scored as a nic mutant. Probably deficient in tryptophan pyrrolase (tryptophan 2,3-dioxygenase) (Fig. 18), but direct evidence is lacking because tryptophan oxygenase cannot be assayed in Neurospora (368). Kynurenine formamidase levels are normal (368). Pyridine nucleotide levels (111).	VIIR	B
sk	VIIR. Right of nt (7 to 17%) (789). Leathery, nonconidiating rapid surface growth (789). "Mucilaginous substrate hyphae" (1088). sk ascospores are slow to mature, but good allele ratios are obtained from crosses held for 3 weeks at 25°C. Female sterile. Allele R2466 called mo-3; alleles Y6821, R2408, and R2529 called moe-1(382, PB).	VIIR	B
wc-1	VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).	VIIR	B