FGSC #7405

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Reporting Genes: chol-2 nit-6 het-8OR ser-6 ad-8

Species: crassa

Allele: 47904 OP4 no# DK42 Y112M343

Alternate Strain Number: 248N46

Depositor: JFL

Linkage Group: VIL L L L L

Mating Type: a

Species Number: 10

Opposite Mating Type: 7404

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-8	VIL. Right of ser-6 (15%) and het-8 (12%). Left of aro-6 (8%) and cpl-1(6 to 11%) (437, 510, 730, PB). Requires adenine; cannot use hypoxanthine (526). Lacks adenylosuccinate synthase (511) (Fig. 8). Fine-structure mapping and intralocus complementation (510-512). Has little hypoxanthine uptake and little hypoxanthine phosphoribosyltransferase; both these effects are partly counteracted in ad-1 ad-8 double mutants (903). Little hypoxanthine phosphoribosyltransferase is also found in mep(3) and mep(10)mutants, q.v. Used to study purine transport (787, 903, and references therein). Called complementation group E.	VIL	B
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B
het-8OR	VIL. Between chol-2 (19%) and ad-8 (12%) (729, 730).Vegetative incompatibility recognized by inhibited duplications from T(T39M777)and subsequently confirmed by heterokaryon tests (729, 730). Photograph of heterozygous duplication colony (729).	VIL	B
nit-6	VIL. Right of chol-2 (6%). Left of ser-7 (10%) and ad-8(17%) (PB; O.C. Yoder, personal communication). Unable to use nitrate or nitrite as a nitrogen source (185). Lacks nitrite reductase (185) (Fig. 19), which is subject to positive nitrogen metabolite repression (186). Affected by nit-2 and MS5 regulator genes (838, 1076a) (see nmr-1). Used to study repression of nitrate reductase (26) and nonenzymatic reduction of nitrite (185). Induced by nitrite (198).	VIL	B
ser-6	VIL. Between nit-6 (10%) and ad-8 (16%) (PB). (544)Responds to serine. Slight response to glycine. Extremely leaky. Scorable by slow, sparse conidiation on minimal slants or auxoanographically (D.D. Perkins, unpublished data). Can be crossed on minimal crossing medium without supplement. Allele DK42 obtained as putative leucine regulatory mutation (544), but a regulatory role is dubious (S.R. Gross, personal communication). Called DK42 (544). Probably allelic with T(VL;VIL)OY325 ser, which has a breakpoint left of lys-5(11%) in VIL, and shows 0/28 recombination with DK42, which it resembles in leakiness. The mutant OY325 grows more profusely than the mutant DK42 on minimal slants (PB).	VIL	B