FGSC #7397

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Reporting Genes: r(Sk-3) acr-7 ser-1

Species: crassa

Allele: P P1676 C127

Alternate Strain Number: 3334

Depositor: BCT

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
acr-7	IIIL. Left of sc (12 to 14%). Linked to thi-4 (7%) (PB). Right of r(Sk-2)-1 (7%) (B.C. Turner, personal communication). Report of VI linkage in reference 818, is incorrect.Resistant to acriflavine (50 µg /ml). Not cross-resistant to 3-aminotriazole or malachite green. Several acr-7 strains have become female infertile after vegetative transfer. (PB)	IIIL	B
ser-1	IIIR. Between sc (4%) and pro-1 (9%) (504). Uses serine or glycine (504). Slightly deficient in serine hydroxymethyltransferase: raised levels of 10-formyltetrahydrofolate synthetase. Lacks ability to incorporate C1 units from glycine. Extracts lack detectable methylfolates. Negligibly deficient in 5,10-methylene tetrahydrofolate reductase. (209). Ascospores from ser-1 x ser-1crosses do not blacken or do so only after a long delay (816). Morphologically normal; abnormal morphology at elevated temperatures that was reported in reference 816 was not due to ser-1 but to scot (810).	IIIR	B
r(Sk-3)	IIIL. Between cum (4%) and acr-7 (17%). Prevents killing by Sk-3 of ascospores that are not themselves Sk-3. Does not confer resistance to killing by Sk-2. Allelism of r(Sk-3) with Sk-3 is not excluded because recombination in this region is blocked when Sk-3 is heterozygous. Recombination is not blocked by r(Sk-3). Found in Neurospora intermedia and introgressed into Neurospora crassa. For reference strains and tester strains for identifying the various killers and genes conferring resistance to killing, see refs. (2127) and (2128) in 2001 Neurospora compendium.	IIIL	B