FGSC #7372

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Reporting Genes: N. crassa wild type Borneo Sk-2K acr-7 leu-1 his-7

Species: crassa

Allele: Borneo P1676 33757 Y152M31

Alternate Strain Number: 3238

Depositor: BCT

Linkage Group: III III III III

Mating Type: A

Species Number: 10

Opposite Mating Type: 7373

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-7	IIIR. Between leu-1 (8 to 20%) and thi-2 (1 to 2%) (2199 1052, PB). (162, 1123)Requires histidine. Blocked before imidazole glycerol phosphate (162, 1123) (Fig. 14). Intralocus recombination (172).	IIIR	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
Sk-2K			B
acr-7	IIIL. Left of sc (12 to 14%). Linked to thi-4 (7%) (PB). Right of r(Sk-2)-1 (7%) (B.C. Turner, personal communication). Report of VI linkage in reference 818, is incorrect.Resistant to acriflavine (50 µg /ml). Not cross-resistant to 3-aminotriazole or malachite green. Several acr-7 strains have become female infertile after vegetative transfer. (PB)	IIIL	B